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Literature summary for 3.2.1.128 extracted from
- Purification and some properties of glycyrrhizinic acid hydrolase from Aspergillus niger GRM3 (1986), Agric. Biol. Chem., 50, 687-692.
No PubMed abstract available
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 150000 | - |
gel filtration | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
GRM 3 | - |
| Aspergillus niger GRM 3 | - |
GRM 3 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus niger |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycyrrhizinic acid + H2O | a pentacyclic triterpene saponin from liquorice roots, best substrate | Aspergillus niger | glycyrrhetinic acid + D-glucuronyl beta-1,2-D-glucuronic acid | - |
? |  |
| glycyrrhizinic acid + H2O | a pentacyclic triterpene saponin from liquorice roots, best substrate | Aspergillus niger GRM 3 | glycyrrhetinic acid + D-glucuronyl beta-1,2-D-glucuronic acid | - |
? | |
| additional information | no substrates are 4-nitrophenyl beta-D-glucuronide, phenolphthalein beta-D-glucuronide | Aspergillus niger | ? | - |
? | |
| additional information | no substrates are 4-nitrophenyl beta-D-glucuronide, phenolphthalein beta-D-glucuronide | Aspergillus niger GRM 3 | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
at pH 5 | Aspergillus niger |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 53 | about 63% of maximal activity at 30°C and about half-maximal activity at 53°C, pH 5 | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.1 | 4.5 | - |
Aspergillus niger |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 4.8 | about half-maximal activity at pH 3 and 4.8 | Aspergillus niger |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6.5 | the enzyme is stable at 40°C | Aspergillus niger |
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