Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.132 extracted from

  • Uchida, Y.; Ohtakara, A.
    Chitosanase from Bacillus species (1988), Methods Enzymol., 161, 501-505.
No PubMed abstract available
Search for more literature for 3.2.1.132

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information 2.0% glycol chitosan Bacillus sp. (in: Bacteria)
additional information
-
 additional information Km: 3.3% soluble chitosan Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 gel filtration Bacillus sp. (in: Bacteria)
41000
-
 1 * 41000, SDS-PAGE Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitosan + H2O Bacillus sp. (in: Bacteria)
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
 K-1
-
Bacillus sp. (in: Bacteria)
-
 No. 7-M
-
Bacillus sp. (in: Bacteria) K-1
-
 K-1
-
Bacillus sp. (in: Bacteria) No. 7-M
-
 No. 7-M
-

Purification (Commentary)

Purification (Comment) Organism
No. 7-M Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
319
-
 after CM-Sephadex C-50 (II) chromatography Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethylcellulose + H2O slow cleavage Bacillus sp. (in: Bacteria) ?
-
 ?
chitosan + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
chitosan + H2O hydrolysis of: soluble chitosan Bacillus sp. (in: Bacteria) additional information glucosamine oligomers with the degree of polymerization from 2 to 8 are produced during hydrolysis of soluble chitosan ?
chitosan + H2O hydrolysis of: glycol chitosan Bacillus sp. (in: Bacteria) additional information glucosamine oligomers with the degree of polymerization from 2 to 8 are produced during hydrolysis of soluble chitosan ?
additional information no activity with chitin (i.e. 100% acetylated chitosan) Bacillus sp. (in: Bacteria) ?
-
 ?
additional information no activity with glycol chitin Bacillus sp. (in: Bacteria) ?
-
 ?
additional information no activity with powdered chitosan Bacillus sp. (in: Bacteria) ?
-
 ?
additional information no activity with cellulose Bacillus sp. (in: Bacteria) ?
-
 ?
additional information no activity with methylcellulose Bacillus sp. (in: Bacteria) ?
-
 ?
additional information no activity with colloidal chitin Bacillus sp. (in: Bacteria) ?
-
 ?
additional information no activity with chitin (i.e. 100% acetylated chitosan) Bacillus sp. (in: Bacteria) K-1 ?
-
 ?
additional information no activity with glycol chitin Bacillus sp. (in: Bacteria) K-1 ?
-
 ?
additional information no activity with powdered chitosan Bacillus sp. (in: Bacteria) K-1 ?
-
 ?
additional information no activity with cellulose Bacillus sp. (in: Bacteria) K-1 ?
-
 ?
additional information no activity with methylcellulose Bacillus sp. (in: Bacteria) K-1 ?
-
 ?
additional information no activity with chitin (i.e. 100% acetylated chitosan) Bacillus sp. (in: Bacteria) No. 7-M ?
-
 ?
additional information no activity with glycol chitin Bacillus sp. (in: Bacteria) No. 7-M ?
-
 ?
additional information no activity with powdered chitosan Bacillus sp. (in: Bacteria) No. 7-M ?
-
 ?
additional information no activity with cellulose Bacillus sp. (in: Bacteria) No. 7-M ?
-
 ?
additional information no activity with methylcellulose Bacillus sp. (in: Bacteria) No. 7-M ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 41000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable below, pH 6.0, 15 min Bacillus sp. (in: Bacteria)
60
-
 pH 6.0, 15 min, 40% loss of activity Bacillus sp. (in: Bacteria)
70
-
 pH 6.0, 15 min, complete loss of activity Bacillus sp. (in: Bacteria)