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Literature summary for 3.2.1.132 extracted from
- Purification and characterization of three chitosanase activities from Bacillus megaterium P1 (1990), Appl. Environ. Microbiol., 56, 844-848.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| chitosan | substrate inhibition, isoform A | Priestia megaterium |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | 0.8 mg/ml chitosan | Priestia megaterium | |
| additional information | - |
additional information | at pH 5.6, 22°C, Km value 0.8 mg per ml, isoform A | Priestia megaterium |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22000 | - |
x * 22000, enzyme C, SDS-PAGE | Priestia megaterium |
| 43000 | - |
x * 43000, enzyme B, SDS-PAGE | Priestia megaterium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitosan + H2O | Priestia megaterium | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Priestia megaterium | - |
- |
- |
| Priestia megaterium | - |
three isoforms, chitosanase A, 43 kilodalton, is highly specific for chitosan and represents the major chitosan-hydrolyzing species. Chitosanases B, 39.5 kilodalton, and C, 22 kilodalton, correspond to minor activities and possess comparable specific activities toward chitosan, chitin, and cellulose | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 3 enzymes: A, B and C | Priestia megaterium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 154.8 | - |
isoform A, pH 5.6, 22°C | Priestia megaterium |
| 163.8 | - |
after Mono-S cation exchange chromatography with 0.06 to 0.08 M KCl | Priestia megaterium |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50% glycerol, stable for at least 6 months | Priestia megaterium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethylcellulose + H2O | substrate for isoforms B, C | Priestia megaterium | ? | - |
? | |
| chitin + H2O | substrate for isoform B, C | Priestia megaterium | ? | - |
? | |
| chitosan + H2O | - |
Priestia megaterium | ? | - |
? | |
| chitosan + H2O | specific substrate, isoform A | Priestia megaterium | ? | - |
? | |
| chitosan + H2O | enzyme A is highly specific for chitosan, enzyme B and C possess comparable specific activities towards chitosan, chitin and cellulose | Priestia megaterium | additional information | glucosamine oligomers, predominantly dimers and trimers | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000, enzyme B, SDS-PAGE | Priestia megaterium |
| ? | x * 22000, enzyme C, SDS-PAGE | Priestia megaterium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Priestia megaterium |
| 50 | - |
enzyme A | Priestia megaterium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
isoform A, stable up to | Priestia megaterium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 6.5 | isoform A | Priestia megaterium |
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