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Literature summary for 3.2.1.132 extracted from

  • Yoon, H.G.; Kim, H.Y.; Kim, H.K.; Hong, B.S.; Shin, D.H.; Cho, H.Y.
    Thermostable chitosanase from Bacillus sp. strain CK4: its purification, characterization, and reaction patterns (2001), Biosci. Biotechnol. Biochem., 65, 802-809.
    View publication on PubMed
Search for more literature for 3.2.1.132

General Stability

General Stability Organism
quite stable in 5% SDS Bacillus sp. (in: Bacteria)
resistant to 6 M urea or 2 M guanidine HCl at 37°C for 30 min Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activity increases about 2.5fold by the addition of 10 mM Co2+ Bacillus sp. (in: Bacteria)
Mn2+ activity increases about 1.4fold by the addition of 10 mM Mn2+ Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
-
 Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitosan + H2O Bacillus sp. (in: Bacteria)
-
 ?
-
 ?
chitosan + H2O Bacillus sp. (in: Bacteria) K-1
-
 ?
-
 ?
chitosan + H2O Bacillus sp. (in: Bacteria) CK4
-
 ?
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
Ethanol
-
 Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
 K-1
-
Bacillus sp. (in: Bacteria)
-
 CK4
-
Bacillus sp. (in: Bacteria) CK4
-
 CK4
-
Bacillus sp. (in: Bacteria) K-1
-
 K-1
-

Purification (Commentary)

Purification (Comment) Organism
CK4 Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
126
-
 after TSK-gel HW-55F gel filtration Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitosan + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
chitosan + H2O
-
 Bacillus sp. (in: Bacteria) K-1 ?
-
 ?
chitosan + H2O
-
 Bacillus sp. (in: Bacteria) CK4 ?
-
 ?
chitosan + H2O enzyme degrades soluble and colloidal chitosan Bacillus sp. (in: Bacteria) additional information
-
 ?
chitosan + H2O enzyme degrades soluble and colloidal chitosan Bacillus sp. (in: Bacteria) K-1 additional information
-
 ?
chitosan + H2O enzyme degrades soluble and colloidal chitosan Bacillus sp. (in: Bacteria) CK4 additional information
-
 ?
glycol chitosan + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
glycol chitosan + H2O
-
 Bacillus sp. (in: Bacteria) K-1 ?
-
 ?
glycol chitosan + H2O
-
 Bacillus sp. (in: Bacteria) CK4 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 29000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 stable for 60 min Bacillus sp. (in: Bacteria)
80
-
 stable for 30 min Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Bacillus sp. (in: Bacteria)