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Literature summary for 3.2.1.132 extracted from

  • Fu, J.Y.; Wu, S.M.; Chang, C.T.; Sung, H.Y.
    Characterization of three chitosanase isozymes isolated from a commercial crude porcine pepsin preparation (2003), J. Agric. Food Chem., 51, 1042-1048.
    View publication on PubMed
Search for more literature for 3.2.1.132

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information KM is 5.2 mg/ml for PSC-I, 4 mg/ml for PSC-II and 5.6 mg/ml for PSC-III Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 enzyme from males and winged females Macrotermes estherae
35000
-
 enzyme from soldiers Macrotermes estherae
37500
-
 gel filtration, isoform PSC-I Sus scrofa
40000
-
 enzyme from workers Macrotermes estherae
40000
-
 1 * 40000, SDS-PAGE Sus scrofa
40600
-
 gel filtration, isoform PSC-II Sus scrofa
43700
-
 gel filtration, isoform PSC-III Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitosan + H2O Sus scrofa
-
 ?
-
 ?
chitosan + H2O Macrotermes estherae
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Macrotermes estherae
-
 workers, soldiers, males, winged females
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
additional information in crude pepsin preparation Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
489.8
-
 isoenzyme PSC-II Sus scrofa
733.5
-
 isoenzyme PSC-III Sus scrofa
742.6
-
 isoenzyme PSC-I Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitosan + H2O
-
 Sus scrofa ?
-
 ?
chitosan + H2O
-
 Macrotermes estherae ?
-
 ?
chitosan + H2O
-
 Macrotermes estherae additional information
-
 ?
chitosan + H2O most effective with 68-88% deacetylated chitosan as substrate Sus scrofa additional information
-
 ?
N,N'',N'''-triacetylchitotriose + H2O
-
 Sus scrofa ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 40000, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23 25 enzyme from soldiers Macrotermes estherae
24
-
 enzyme from males Macrotermes estherae
24 26 enzyme from workers Macrotermes estherae
25
-
 enzyme from winged females Macrotermes estherae
30
-
 PSC-III Sus scrofa
40
-
 PSC-I and PSC-II Sus scrofa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 70 all three isoforms are stable in this range for 30 min Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3
-
 chitosan 40°C, pH 4, isoform PSC-II Sus scrofa
5.9
-
 chitosan 40°C, pH 4, isoform PSC-I Sus scrofa
6.2
-
 chitosan 40°C, pH 4, isoform PSC-III Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 isoenzyme PSC-III Sus scrofa
4.9
-
 enzyme from males Macrotermes estherae
5
-
 isoenzymes PSC-I and PSC-II Sus scrofa
5.3
-
 enzyme from winged females Macrotermes estherae
5.8
-
 enzyme from soldiers Macrotermes estherae
6.2
-
 enzyme from workers Macrotermes estherae

pH Range

pH Minimum pH Maximum Comment Organism
6 9 isoenzyme PSC-III is stable in this range Sus scrofa

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 7 isoenzyme PSC-II is stable in this range Sus scrofa
5 8 isoenzyme PSC-I is stable in this range Sus scrofa

pI Value

Organism Comment pI Value Maximum pI Value
Sus scrofa isoelectrofocusing, PSC-III
-
 4.4
Sus scrofa isoelectrofocusing, PSC-II 4.7 4.6
Sus scrofa isoelectrofocusing, PSC-I
-
 4.9