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Literature summary for 3.2.1.132 extracted from

  • Su, C.; Wang, D.; Yao, L.; Yu, Z.
    Purification, characterization, and gene cloning of a chitosanase from Bacillus species strain S65 (2006), J. Agric. Food Chem., 54, 4208-4214.
    View publication on PubMed
Search for more literature for 3.2.1.132

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
CaCl2 10 mM, 48% inhibition Bacillus sp. (in: Bacteria)
CoCl2 10 mM, 33% inhibition Bacillus sp. (in: Bacteria)
CuCl2 10 mM, 69% inhibition Bacillus sp. (in: Bacteria)
FeCl2 1 mM, 80.7% inhibition Bacillus sp. (in: Bacteria)
FeCl3 1 mM, 84.8% inhibition Bacillus sp. (in: Bacteria)
ZnCl2 10 mM, 42.8% inhibition Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
CoCl2 5 mM, 15% enhancement of activity Bacillus sp. (in: Bacteria)
CuCl2 1 mM, 19% enhancement of activity Bacillus sp. (in: Bacteria)
MnCl2 1 mM, 41.2% enhancement of activity Bacillus sp. (in: Bacteria)
ZnCl2 1 mM, 24.3% enhancement of activity Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 x * 45000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) S65
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Source Tissue

Source Tissue Comment Organism Textmining
culture medium enzyme is constitutively produced Bacillus sp. (in: Bacteria)
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
93.6
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl cellulose + H2O 5.3% of the activity with soluble chitosan Bacillus sp. (in: Bacteria) ?
-
 ?
carboxymethyl cellulose + H2O 5.3% of the activity with soluble chitosan Bacillus sp. (in: Bacteria) S65 ?
-
 ?
chitosan + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
chitosan + H2O
-
 Bacillus sp. (in: Bacteria) S65 ?
-
 ?
additional information the enzyme cannot hydrolyze colloidal chitin and crystalline cellulose Bacillus sp. (in: Bacteria) ?
-
 ?
additional information the enzyme cannot hydrolyze colloidal chitin and crystalline cellulose Bacillus sp. (in: Bacteria) S65 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
55 70 55°C: about 85% of maximal activity, 70°C: about 90% of maximal activity Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 pH 6, 1 h, stable below Bacillus sp. (in: Bacteria)
50
-
 pH 6, 1 h, about 50% loss of activity Bacillus sp. (in: Bacteria)
65
-
 pH 6, 10 min, about 90% loss of activity Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 6.5 28°C, 60 min, stable Bacillus sp. (in: Bacteria)

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus sp. (in: Bacteria)
-
-
 6