Literature summary for 3.2.1.132 extracted from

Shimosaka, M.; Sato, K.; Nishiwaki, N.; Miyazawa, T.; Okazaki, M.
Analysis of essential carboxylic amino acid residues for catalytic activity of fungal chitosanases by site-directed mutagenesis (2005), J. Biosci. Bioeng., 100, 545-550.

Search for more literature for 3.2.1.132

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Saccharomyces cerevisiae	Fusarium solani

Protein Variants

Protein Variants	Comment	Organism
D175E	48.3% of wild-type activity with acetylated chitosan as substrate (acetylation degree: 30%)	Fusarium solani
D175N	inactive mutant enzyme	Fusarium solani
D212N	37.9% of wild-type activity with acetylated chitosan as substrate (acetylation degree: 30%)	Fusarium solani
E188D	23.6% of wild-type activity with acetylated chitosan as substrate (acetylation degree: 30%)	Fusarium solani
E188Q	inactive mutant enzyme	Fusarium solani

Organism

Organism	UniProt	Comment	Textmining
Fusarium solani	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
chitosan + H2O	degree of acetylation is 30%	Fusarium solani	?	-	?

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Release 2023.1 (January 2023)