Literature summary for 3.2.1.132 extracted from

Adachi, W.; Sakihama, Y.; Shimizu, S.; Sunami, T.; Fukazawa, T.; Suzuki, M.; Yatsunami, R.; Nakamura, S.; Takenaka, A.
Crystal structure of family GH-8 chitosanase with subclass II specificity from Bacillus sp. K17 (2004), J. Mol. Biol., 343, 785-795.

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Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of wild-type enzyme and mutant enzymes E122Q, D183N and E309Q in Escherichia coli	Bacillus sp. (in: Bacteria)

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure determined at 1.5 A resolution in the active form and at 2.0 A resolution in the inactive form. Hanging-drop vapor-diffusion method	Bacillus sp. (in: Bacteria)

Protein Variants

Protein Variants	Comment	Organism
D183N	0.0403% of wild-type activity	Bacillus sp. (in: Bacteria)
E122Q	0.00965% of wild-type activity	Bacillus sp. (in: Bacteria)
E309Q	0.0105% of wild-type activity	Bacillus sp. (in: Bacteria)

Organism

Organism	UniProt	Comment	Textmining
Bacillus sp. (in: Bacteria)	-	-	-
Bacillus sp. (in: Bacteria) K17	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
chitosan + H2O	-	Bacillus sp. (in: Bacteria)	?	-	?
chitosan + H2O	-	Bacillus sp. (in: Bacteria) K17	?	-	?

Synonyms

Synonyms	Comment	Organism
ChoK	-	Bacillus sp. (in: Bacteria)

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Release 2023.1 (January 2023)