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Literature summary for 3.2.1.132 extracted from
- Purification and characterization of a bifunctional enzyme with chitosanase and cellulase activity from commercial cellulase (2006), Biochem. Eng. J., 30, 82-87.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 2.5 mM, 16% increase in activity | Trichoderma viride |  |
| Mn2+ | 3 mM, 18% increase in activity | Trichoderma viride | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 1 mM, 20% loss of activity | Trichoderma viride | |
| Hg2+ | 1 mM, 80% loss of activity | Trichoderma viride | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
chitosan | Km value is 10 mg/ml | Trichoderma viride | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
gel filtration | Trichoderma viride |
| 66000 | - |
1 * 66000, SDS-PAGE | Trichoderma viride |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichoderma viride | - |
commercial preparation of cellulase, isolation of a bifunctional chitosanase/cellulase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isolation of a bifunctional chitosanase/cellulase from commercial prepapration of cellulase | Trichoderma viride |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.402 | - |
pH 5.2, 60°C | Trichoderma viride |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitosan + H2O | - |
Trichoderma viride | glucosamine oligomer + N-acetyl-glucosamine oligomer | hydrolysates are oligomers with one to four glucosamine residues and some oligomers with longer chain length | ? | |
| additional information | enzyme cleaves both glucosamine-glucosamine and N-acetylglucosamine-glucosamine linkages of chitosan. Enzyme additionally has cellulase activity for carboxymethylcellulose | Trichoderma viride | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 66000, SDS-PAGE | Trichoderma viride |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Trichoderma viride |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | stable for 2 h | Trichoderma viride |
| 80 | - |
15 min, almost complete loss of activity | Trichoderma viride |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | - |
chitosan hydrolysis | Trichoderma viride |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | 30°C, stable for at least 24 h | Trichoderma viride |
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