Literature summary for 3.2.1.132 extracted from

Roy, S.; Fortin, M.; Gagnon, J.; Ghinet, M.G.; LeHoux, J.; Dupuis, G.; Brzezinski, R.
Quantitative fluorometric analysis of the protective effect of chitosan on thermal unfolding of catalytically active native and genetically-engineered chitosanases (2007), Biochim. Biophys. Acta, 1774, 975-984.

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Application

Application	Comment	Organism
analysis	fluorimetric determination of transition temperature is a reliable method for rapid assessment of the thermal behaviour of chitosanases and applicable to structure-function studies	Streptomyces coelicolor
analysis	fluorimetric determination of transition temperature is a reliable method for rapid assessment of the thermal behaviour of chitosanases and applicable to structure-function studies	Streptomyces sp.

Protein Variants

Protein Variants	Comment	Organism
additional information	consensus enzyme mutant designed by multiple amino acid substitutions. Increase in transition temperature	Streptomyces sp.

Organism

Organism	UniProt	Comment	Textmining
Streptomyces coelicolor	-	strain strain A3	-
Streptomyces sp.	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
chitosan + H2O	substrate with average molecular weight of 220 kDA, 85% deacetylated	Streptomyces coelicolor	chitosan oligosaccharides	-	?
chitosan + H2O	substrate with average molecular weight of 220 kDA, 85% deacetylated	Streptomyces sp.	chitosan oligosaccharides	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
43.4	-	transition temperature in absence of chitosan	Streptomyces coelicolor
54	-	wild-type, transition temperature in absence of chitosan	Streptomyces sp.
61	-	both wild-type and mutants A104L, K164R are active over 90 min	Streptomyces sp.
68.6	-	transition temperature in presence of chitosan at 6 g/l	Streptomyces coelicolor
69.5	-	wild-type, transition temperature in presence of chitosan at 6 g/l	Streptomyces sp.
81.1	-	consensus mutant, transition temperature in presence of chitosan at 6 g/l	Streptomyces sp.
83.3	-	mutant A104L, transition temperature in presence of chitosan at 6 g/l	Streptomyces sp.
85.1	-	mutant K164R, transition temperature in presence of chitosan at 6 g/l	Streptomyces sp.

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Release 2023.1 (January 2023)