Application | Comment | Organism |
---|---|---|
industry | a cheap chitosanase for large-scale chitosan oligosaccharide production in industry | Bacillus sp. (in: Bacteria) |
Cloned (Comment) | Organism |
---|---|
amplified DNA fragment ligated into a pGEM T-easy vector, subsequently subcloned from the T-vector into a pET vector, expressed as 6 x His tag fused at its N-terminal in the Escherichia coli strain BL21(DE3) as a soluble and active form | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity by immobilized metal-ion affinity chromatography | Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
between 270-290 U/mg, recombinant chitosanase | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitosan + H2O | 1 g of enzyme can hydrolyze about 100 kg of chitosan | Bacillus sp. (in: Bacteria) | chitosan oligosaccharides | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | its half-life is only several min at 60°C. At lower temperatures, the enzyme is quite stable, its half-life is extended to several hours at 50 and 40°C. No detectable activity loss at room temperature for several days | Bacillus sp. (in: Bacteria) |