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Literature summary for 3.2.1.132 extracted from

  • Wang, S.L.; Wu, P.C.; Liang, T.W.
    Utilization of squid pen for the efficient production of chitosanase and antioxidants through prolonged autoclave treatment (2009), Carbohydr. Res., 344, 979-984.
    View publication on PubMed
Search for more literature for 3.2.1.132

Application

Application Comment Organism
food industry has potential in the production of functional foods Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 5 mM inhibits by 15%, in 50 mM phosphate buffer, pH 7, for 30 min at 37°C Bacillus sp. (in: Bacteria)
Ca2+ 5 mM inhibits by 10%, in 50 mM phosphate buffer, pH 7, for 30 min at 37°C Bacillus sp. (in: Bacteria)
Cu2+ 5 mM inhibits by 41%, in 50 mM phosphate buffer, pH 7, for 30 min at 37°C Bacillus sp. (in: Bacteria)
EDTA 5 mM inhibits by 10%, in 50 mM phosphate buffer, pH 7, for 30 min at 37°C Bacillus sp. (in: Bacteria)
Fe2+ 5 mM inhibits by 77%, in 50 mM phosphate buffer, pH 7, for 30 min at 37°C Bacillus sp. (in: Bacteria)
Mg2+ 5 mM inhibits by 14%, in 50 mM phosphate buffer, pH 7, for 30 min at 37°C Bacillus sp. (in: Bacteria)
Mn2+ 5 mM inhibits by 17%, in 50 mM phosphate buffer, pH 7, for 30 min at 37°C Bacillus sp. (in: Bacteria)
additional information in the presence of 2% Tween 20, Tween 40, Triton X-100 (non-ionic surfactant), and 2 mM SDS (anionic surfactant), the enzyme retains almost original activity Bacillus sp. (in: Bacteria)
Zn2+ 5 mM inhibits by 18%, in 50 mM phosphate buffer, pH 7, for 30 min at 37°C Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 gel filtration Bacillus sp. (in: Bacteria)
29000
-
 1 * 29000, SDS–PAGE Bacillus sp. (in: Bacteria)
31480
-
 sequence analysis Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) TKU004
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by gel filtration, about 527fold with overall activity yield of 20%, to homogeneity Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.005316
-
 culture supernatant Bacillus sp. (in: Bacteria)
2.8
-
 527fold purified enzyme Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin (beta-type) + H2O 2% relative activity Bacillus sp. (in: Bacteria) ?
-
 ?
chitin (beta-type) + H2O 2% relative activity Bacillus sp. (in: Bacteria) TKU004 ?
-
 ?
colloidal chitin + H2O 6% relative activity Bacillus sp. (in: Bacteria) ?
-
 ?
colloidal chitin + H2O 6% relative activity Bacillus sp. (in: Bacteria) TKU004 ?
-
 ?
additional information chitosanase has antioxidant activity. Squid pen powder is a carbon/nitrogen source for chitosanase production. Autoclave treatment of squid pen powder for 45 min remarkably promotes enzyme productivity, production of chitosanase is highest when 3% squid pen powder is used. No acitivity with chitosan (80% degree of deacetylation), chitosan (73% degree of deacetylation), chitosan (60% degree of deacetylation) and chitin (alpha-type) Bacillus sp. (in: Bacteria) ?
-
 ?
additional information chitosanase has antioxidant activity. Squid pen powder is a carbon/nitrogen source for chitosanase production. Autoclave treatment of squid pen powder for 45 min remarkably promotes enzyme productivity, production of chitosanase is highest when 3% squid pen powder is used. No acitivity with chitosan (80% degree of deacetylation), chitosan (73% degree of deacetylation), chitosan (60% degree of deacetylation) and chitin (alpha-type) Bacillus sp. (in: Bacteria) TKU004 ?
-
 ?
soluble chitosan + H2O activity decreases with the degree of deacetylation of chitosan. The enzyme hydrolyzes chitosan with 82-100% degree of deacetylation. Bacillus sp. (in: Bacteria) ?
-
 ?
soluble chitosan + H2O activity decreases with the degree of deacetylation of chitosan. The enzyme hydrolyzes chitosan with 82-100% degree of deacetylation. Bacillus sp. (in: Bacteria) TKU004 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 29000, SDS–PAGE Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 40 maintains its initial activity from 25 to 40°C but is completely inactivated at 80°C Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 7
-
 Bacillus sp. (in: Bacteria)