Literature summary for 3.2.1.132 extracted from

Yao, Y.Y.; Shrestha, K.L.; Wu, Y.J.; Tasi, H.J.; Chen, C.C.; Yang, J.M.; Ando, A.; Cheng, C.Y.; Li, Y.K.
Structural simulation and protein engineering to convert an endo-chitosanase to an exo-chitosanase (2008), Protein Eng. Des. Sel., 21, 561-566.

Search for more literature for 3.2.1.132

Cloned(Commentary)

Cloned (Comment)	Organism
chimeric chitosanase and wild-type Csn overexpressed from vector pET22b(+) in Escherichia coli strain BL21 (DE3)	Niallia circulans

Protein Variants

Protein Variants	Comment	Organism
additional information	chimeric chitosanase produced by inserting two peptide loops, each containing a cysteine residue, in opposite walls of the substrate-binding cleft. The two cysteine residues form a disulfide bond crossing the protruding loop, which may alter the binding topology of the substrate and consequently convert the endo-chitosanase into an exo-type enzyme	Niallia circulans

Organism

Organism	UniProt	Comment	Textmining
Niallia circulans	P33673	-	-
Niallia circulans MH-K1	P33673	-	-

Purification (Commentary)

Purification (Comment)	Organism
chimeric chitosanase and wild-type Csn purified nearly to homogeneity by cation-exchange chromatography	Niallia circulans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
chitosan + H2O	chitosan with 85% degree of deacetylation. Inserting two surface loops, the endo-type chitosanase Csn is converted into an exo-type chitosanase. The chimeric chitosanase has 3% of wild-type activity and GlcNbeta(1-4)GlcN is the dominant product, whereas a mixture of GlcNbeta(1-4)GlcN, GlcNbeta(1-4)GlcNbeta(1-4)GlcN and GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcN is obtained with the wild-type endo-chitosanase. Chimeric Csn catalyzes the hydrolysis of chitosan with a smaller rate of viscosity decrease than the wild-type	Niallia circulans	GlcNbeta(1-4)GlcN + GlcNbeta(1-4)GlcNbeta(1-4)GlcN + GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcN	-	?
chitosan + H2O	chitosan with 85% degree of deacetylation. Inserting two surface loops, the endo-type chitosanase Csn is converted into an exo-type chitosanase. The chimeric chitosanase has 3% of wild-type activity and GlcNbeta(1-4)GlcN is the dominant product, whereas a mixture of GlcNbeta(1-4)GlcN, GlcNbeta(1-4)GlcNbeta(1-4)GlcN and GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcN is obtained with the wild-type endo-chitosanase. Chimeric Csn catalyzes the hydrolysis of chitosan with a smaller rate of viscosity decrease than the wild-type	Niallia circulans MH-K1	GlcNbeta(1-4)GlcN + GlcNbeta(1-4)GlcNbeta(1-4)GlcN + GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcN	-	?

Synonyms

Synonyms	Comment	Organism
Csn	-	Niallia circulans
endo-chitosanase	-	Niallia circulans

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Release 2023.1 (January 2023)