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Literature summary for 3.2.1.132 extracted from
- Enhancement of the activity and pH-performance of chitosanase from Bacillus cereus strains by DNA shuffling (2009), Biotechnol. Lett., 31, 1463-1467.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of GST-tagged shuffled mutant enzymes Y18 and Y20 in Escherichia coli strain BL21(DE3) | Bacillus cereus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| I13T/A87V | DNA shuffling of the genes from strains KNUC51 and KNUC55, the shuffled product YM18 shows higher activity than the parents at 40°C. The specific activity of YM18 is enhanced 250% compared to the parents | Bacillus cereus |
| K66R/N352S | DNA shuffling of the genes from strains KNUC51 and KNUC55, the shuffled product YM20 shows higher activity than the parents at 40°C. The specific activity of YM20 is enhanced 350% compared to the parents, YM20 exhibits a shift of the optimal pH level from pH 5.5 to pH 6.5 | Bacillus cereus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
x * 50000, recombinant detagged enzymes, SDS-PAGE, x * 81000, recombinant GST-tagged enzymes, SDS-PAGE | Bacillus cereus |
| 81000 | - |
x * 50000, recombinant detagged enzymes, SDS-PAGE, x * 81000, recombinant GST-tagged enzymes, SDS-PAGE | Bacillus cereus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | - |
strains KNUC51 and KNUC55, genes csn | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GST-tagged shuffled mutant enzymes Y18 and Y20 from EScherichia coli strain BL231(DE3) by glutathione affinity chromatography. The tags are cleaved off by thrombin | Bacillus cereus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitosan + H2O | soluble chitosan | Bacillus cereus | ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000, recombinant detagged enzymes, SDS-PAGE, x * 81000, recombinant GST-tagged enzymes, SDS-PAGE | Bacillus cereus |
| More | structure comparisons of wild-type and mutant enzymes, overview | Bacillus cereus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the enzyme belongs to the glycohydrolase family 8 | Bacillus cereus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
wild-type enzyme | Bacillus cereus |
| 50 | - |
mutant YM18 | Bacillus cereus |
| 70 | - |
mutant YM20 | Bacillus cereus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
broader pH activity profile for YM20, in contrast to the pH activity profile of YM18, which shows the same pH optimum as the wild-type enzyme at pH 5.5 | Bacillus cereus |
| 5.5 | - |
wild-type enzyme | Bacillus cereus |
| 6.5 | - |
mutant YM20 | Bacillus cereus |
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