Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 89% inhibition at 1 mM, 79% at 0.5 mM | Mucor circinelloides | |
Ba2+ | 11% inhibition at 1 mM | Mucor circinelloides | |
Cd2+ | 13% inhibition at 1 mM | Mucor circinelloides | |
Co2+ | 13% inhibition at 1 mM | Mucor circinelloides | |
Cu2+ | 92% inhibition at 1 mM, 82% at 0.5 mM | Mucor circinelloides | |
EDTA | 17% inhibition at 1% w/v, 82% at 5% w/v | Mucor circinelloides | |
Fe2+ | 26% inhibition at 1 mM, 9% at 0.5 mM | Mucor circinelloides | |
Hg2+ | complete inhibition at 1 mM | Mucor circinelloides | |
Pb2+ | 14% inhibition at 1 mM, 8% at 0.5 mM | Mucor circinelloides | |
SDS | 82% inhibition at 1% w/v | Mucor circinelloides | |
Sn2+ | 20% inhibition at 1 mM, 9% at 0.5 mM | Mucor circinelloides | |
Triton X-100 | 80% inhibition at 1% w/v | Mucor circinelloides | |
Tween 20 | 72% inhibition at 1% w/v | Mucor circinelloides | |
Tween 80 | 79% inhibition at 1% w/v | Mucor circinelloides | |
Zn2+ | 24% inhibition at 1 mM, 9% at 0.5 mM | Mucor circinelloides |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Mucor circinelloides | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Mucor circinelloides | |
Mg2+ | activates | Mucor circinelloides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
x * 42000, SDS-PAGE | Mucor circinelloides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mucor circinelloides | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 23fold from mycelium by bacitracin affinity chromatography and gel filtration | Mucor circinelloides |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | air-dried and defatted | Mucor circinelloides | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitosan + H2O | the chitosanase is an endo-type enzyme, the preferred substrates of chitosanase are chitosan preparations with a high degree of deacetylation, substrate is chitosan 66-97% deacetylated | Mucor circinelloides | ? | - |
? | |
additional information | The enzyme might be identical with a lipase that co-purifies with the chitosanase. No activity for colloidal chitin, Na-CMC and starch | Mucor circinelloides | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, SDS-PAGE | Mucor circinelloides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Mucor circinelloides |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | 15-20% of maximal activity at 20°C, 30% at 50°C, temperature profile, overview | Mucor circinelloides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6 | - |
Mucor circinelloides |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | 20% of maximal activity at pH 4.0, 10% at pH 7.0, pH profile, overview | Mucor circinelloides |