Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Microbacterium sp. |
Crystallization (Comment) | Organism |
---|---|
in complex with hexaglucosamine, hanging drop vapor diffusion method, using 0.05 M KH2PO4 and 23% (w/v) PEG 8000 using 0.7 M NaCl as the reservoir at 15°C | Microbacterium sp. |
Protein Variants | Comment | Organism |
---|---|---|
D235A | the mutant shows about 18% of wild type activity | Microbacterium sp. |
D43A | inactive | Microbacterium sp. |
E25A | inactive | Microbacterium sp. |
H203A | the mutant shows about 3% of wild type activity | Microbacterium sp. |
S27A | the mutant shows about 80% of wild type activity | Microbacterium sp. |
T58A | the mutant shows about 20% of wild type activity | Microbacterium sp. |
Y37F | the mutant shows about 5% of wild type activity | Microbacterium sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitosan + H2O | Microbacterium sp. | - |
chitosan oligomers | chitodimer and chitotrimer as main products, a monoacetyl chitodimer and a monoacetyl chitotrimer but no significant amount of monomer glucosamine or chitotetramer is identified | ? | |
chitosan + H2O | Microbacterium sp. OU01 | - |
chitosan oligomers | chitodimer and chitotrimer as main products, a monoacetyl chitodimer and a monoacetyl chitotrimer but no significant amount of monomer glucosamine or chitotetramer is identified | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Microbacterium sp. | A7KBW5 | - |
- |
Microbacterium sp. OU01 | A7KBW5 | - |
- |
Purification (Comment) | Organism |
---|---|
glutathione-Sepharose 4B bead chromatography, and Sephacryl S-100 gel filtration | Microbacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitohexaose + H2O | - |
Microbacterium sp. | ? | - |
? | |
chitohexaose + H2O | - |
Microbacterium sp. OU01 | ? | - |
? | |
chitosan + H2O | - |
Microbacterium sp. | chitosan oligomers | chitodimer and chitotrimer as main products, a monoacetyl chitodimer and a monoacetyl chitotrimer but no significant amount of monomer glucosamine or chitotetramer is identified | ? | |
chitosan + H2O | - |
Microbacterium sp. OU01 | chitosan oligomers | chitodimer and chitotrimer as main products, a monoacetyl chitodimer and a monoacetyl chitotrimer but no significant amount of monomer glucosamine or chitotetramer is identified | ? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
42.5 | - |
melting temperature of the wild type enzyme | Microbacterium sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
205 | - |
chitosan | at pH 5.6 and 37°C | Microbacterium sp. |