Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.132 extracted from

  • Liang, T.W.; Chen, Y.Y.; Pan, P.S.; Wang, S.L.
    Purification of chitinase/chitosanase from Bacillus cereus and discovery of an enzyme inhibitor (2014), Int. J. Biol. Macromol., 63, 8-14.
    View publication on PubMed
Search for more literature for 3.2.1.132

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 8.1% residual activity at 5 mM Bacillus cereus
EDTA 73.4% residual activity at 5 mM Bacillus cereus
Fe2+ 18.7% residual activity at 5 mM Bacillus cereus
Mn2+ 2.3% residual activity at 5 mM Bacillus cereus
additional information not inhibited by phenylmethylsulfonyl fluoride, TKUPSP018, TKUPSP073, TKUPSP011, TKUPSP035, TKUPSP080, and TKUPSP062 Bacillus cereus
SDS 51.3% residual activity at 2 mM Bacillus cereus
TKUPSP017 chitosanase is inhibited completely by 0.05% (w/v) TKUPSP017 Bacillus cereus
TKUPSP074 chitosanase is inhibited completely by 0.05% (w/v) TKUPSP074 Bacillus cereus
TritonX-100 48.4% residual activity at 1% (v/v) Bacillus cereus
Tween 20 57.2% residual activity at 0.5% (v/v) Bacillus cereus
Tween 40 51.2% residual activity at 1% (v/v) Bacillus cereus
Zn2+ 41.5% residual activity at 5 mM Bacillus cereus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 x * 43000, SDS-PAGE Bacillus cereus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitosan + H2O Bacillus cereus 100% activity with chitosan of 60% degrees of deacetylation ?
-
 ?
chitosan + H2O Bacillus cereus TKU030 100% activity with chitosan of 60% degrees of deacetylation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-
Bacillus cereus TKU030
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Sepharose CL-6B column chromatography, and Sephacryl S-100 gel filtration Bacillus cereus

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.002
-
 culture supernatant, at pH 4.0 and 40°C Bacillus cereus
0.177
-
 after 88.5fold purification, at pH 4.0 and 40°C Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-chitin + H2O 11.8% activity compared to chitosan Bacillus cereus ?
-
 ?
alpha-chitin + H2O 11.8% activity compared to chitosan Bacillus cereus TKU030 ?
-
 ?
beta-chitin + H2O 68.7% activity compared to chitosan Bacillus cereus ?
-
 ?
beta-chitin + H2O 68.7% activity compared to chitosan Bacillus cereus TKU030 ?
-
 ?
carboxylmethyl cellulose + H2O 61.9% activity compared to chitosan Bacillus cereus ?
-
 ?
carboxylmethyl cellulose + H2O 61.9% activity compared to chitosan Bacillus cereus TKU030 ?
-
 ?
chitosan + H2O 100% activity with chitosan of 60% degrees of deacetylation Bacillus cereus ?
-
 ?
chitosan + H2O 100% activity with chitosan of 60% degrees of deacetylation Bacillus cereus TKU030 ?
-
 ?
additional information no activity with colloidal chitin Bacillus cereus ?
-
 ?
additional information no activity with colloidal chitin Bacillus cereus TKU030 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Bacillus cereus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Bacillus cereus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 50 the enzyme is stable between 25 and 50°C, maintains its initial activity from 25 to 40°C and has 50% of its activity at 50°C, and is inactivated at 80°C Bacillus cereus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Bacillus cereus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 7 the enzyme is stable between pH 3.0 and 7.0 Bacillus cereus