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Literature summary for 3.2.1.132 extracted from

  • Hsu, S.; Chung, Y.; Chang, C.; Sung, H.
    Purification and characterization of two chitosanase isoforms from the sheaths of bamboo shoots (2012), J. Agric. Food Chem., 60, 649-657.
    View publication on PubMed
Search for more literature for 3.2.1.132

Activating Compound

Activating Compound Comment Organism Structure
diethyl dicarbonate isoform A shows 111% activity at 2.5 mM Bambusa oldhamii
p-hydroxymercuribenzoate isoform A shows 112% activity at 0.5 mM Bambusa oldhamii

Inhibitors

Inhibitors Comment Organism Structure
2,4-Dinitro-1-fluorobenzene isoform A shows 95% residual activity at 0.625 mM, isoform B shows 84% residual activity at 0.625 mM Bambusa oldhamii
Ag+ isoform A shows 91% residual activity at 1 mM, isoform B shows 86% residual activity at 1 mM Bambusa oldhamii
ethyl acetimidate isoform B shows 56% residual activity at 250 mM Bambusa oldhamii
Hg2+ isoform B shows 77% residual activity at 0.25 mM Bambusa oldhamii
additional information not inhibited by diethyl dicarbonate, 1,2-cyclohexanedione, and p-hydroxymercuribenzoate Bambusa oldhamii
N-Acetylimidazole isoform A shows 96% residual activity at 5 mM, isoform B shows 84% residual activity at 5 mM Bambusa oldhamii
N-bromosuccinimide isoform A shows 12% residual activity at 2.5 mM, isoform B is completely inhibited at 2.5 mM Bambusa oldhamii
phenylmethylsulfonyl fluoride isoform B shows 86% residual activity at 2.5 mM Bambusa oldhamii
Woodward's reagent K N-ethyl-5-phenylisoazoline-3'-sulfonate, isoform A shows 95% residual activity at 50 mM, isoform B shows 1% residual activity at 50 mM Bambusa oldhamii

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ isoform B shows 117% residual activity at 1 mM Bambusa oldhamii
additional information not influenced by Cu2+, Mg2+, K+, and EDTA Bambusa oldhamii
Na+ isoform A shows 111% activity at 5 mM Bambusa oldhamii
Zn2+ isoform B shows 111% residual activity at 1 mM Bambusa oldhamii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16400
-
 x * 16400, isoform B, SDS-PAGE Bambusa oldhamii
16400
-
 x * 24500 + x * 16400, purified isoform A is composed of a major and a minor chitosanase isoform, SDS-PAGE Bambusa oldhamii
24500
-
 x * 24500 + x * 16400, purified isoform A is composed of a major and a minor chitosanase isoform, SDS-PAGE Bambusa oldhamii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitosan + H2O Bambusa oldhamii chitosan polymers with various degrees of deacetylation (10-94%) are susceptible to hydrolysis by both isoforms. Polymers with 50-70% deacetylation are most susceptible to hydrolysis by isoform A and polymers with 40-80% deacetylation are most susceptible to isoform B D-glucosamine + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bambusa oldhamii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Sephacryl S-100 gel filtration Bambusa oldhamii

Source Tissue

Source Tissue Comment Organism Textmining
shoot
-
 Bambusa oldhamii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0065
-
 crude extract, at pH and °C Bambusa oldhamii
0.243
-
 isoform B, after 115.1fold purification, at pH 4.5 and 50°C Bambusa oldhamii
0.753
-
 isoform A, after 115.1fold purification, at pH 4.5 and 50°C Bambusa oldhamii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl chitin + H2O isoform A shows 41.8% activity and isoform B 17.2% activity compared to chitosan Bambusa oldhamii ?
-
 ?
chitin + H2O isoform A shows 7.8% activity and isoform B 10.8% activity compared to chitosan Bambusa oldhamii ?
-
 ?
chitosan + H2O chitosan polymers with various degrees of deacetylation (10-94%) are susceptible to hydrolysis by both isoforms. Polymers with 50-70% deacetylation are most susceptible to hydrolysis by isoform A and polymers with 40-80% deacetylation are most susceptible to isoform B Bambusa oldhamii D-glucosamine + ?
-
 ?
glycol chitin + H2O isoform A shows 29.7% activity and isoform B 14.1% activity compared to chitosan Bambusa oldhamii ?
-
 ?

Subunits

Subunits Comment Organism
? x * 16400, isoform B, SDS-PAGE Bambusa oldhamii
? x * 24500 + x * 16400, purified isoform A is composed of a major and a minor chitosanase isoform, SDS-PAGE Bambusa oldhamii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 isoform B Bambusa oldhamii
70
-
 isoform A Bambusa oldhamii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 80 isoform A is stable between 30 and 80°C, whereas isoform B is stable between 30 and 50°C after 60 min incubation Bambusa oldhamii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3 4
-
 Bambusa oldhamii

pH Range

pH Minimum pH Maximum Comment Organism
3 6 both purified isoforms A and B retain approximately 70-100% relative activity between pH 3.0 and 6.0 but rapidly lose activity above pH 6.0 Bambusa oldhamii

pI Value

Organism Comment pI Value Maximum pI Value
Bambusa oldhamii isoform A, isoelectric focusing
-
 4.3
Bambusa oldhamii isoform B, isoelectric focusing
-
 9.2