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Literature summary for 3.2.1.132 extracted from

  • Doan, C.T.; Tran, T.N.; Nguyen, V.B.; Nguyen, A.D.; Wang, S.L.
    Production of a thermostable chitosanase from shrimp heads via Paenibacillus mucilaginosus TKU032 conversion and its application in the preparation of bioactive chitosan oligosaccharides (2019), Mar. Drugs, 17, 217.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.132

Application

Application Comment Organism
food industry chitosanase from Paenibacillus mucilaginosus TKU032 may have potential applications in production of bioactive chitosan oligosaccharides for the food and pharmaceutical industries Paenibacillus mucilaginosus
pharmacology chitosanase from Paenibacillus mucilaginosus TKU032 may have potential applications in production of bioactive chitosan oligosaccharides for the food and pharmaceutical industries Paenibacillus mucilaginosus

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 5 mM, 38% loss of activity Paenibacillus mucilaginosus
Ca2+ 5 mM, 23% loss of activity Paenibacillus mucilaginosus
Cu2+ 5 mM, 26% loss of activity Paenibacillus mucilaginosus
EDTA 5 mM, 16% loss of activity Paenibacillus mucilaginosus
Fe2+ 5 mM, 36% loss of activity Paenibacillus mucilaginosus
Mg2+ 5 mM, 16% loss of activity Paenibacillus mucilaginosus
Na+ 5 mM, 8% loss of activity Paenibacillus mucilaginosus
Zn2+ 5 mM, 23% loss of activity Paenibacillus mucilaginosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
 SDS-PAGE Paenibacillus mucilaginosus

Organism

Organism UniProt Comment Textmining
Paenibacillus mucilaginosus
-
-
-
Paenibacillus mucilaginosus TKU032
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Paenibacillus mucilaginosus

Source Tissue

Source Tissue Comment Organism Textmining
culture condition medium containing shrimp heads as the sole carbon and nitrogen source Paenibacillus mucilaginosus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.03
-
 pH 7, 37°C Paenibacillus mucilaginosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellulose + H2O
-
 Paenibacillus mucilaginosus ?
-
 ?
cellulose + H2O
-
 Paenibacillus mucilaginosus TKU032 ?
-
 ?
chitin + H2O
-
 Paenibacillus mucilaginosus ?
-
 ?
chitin + H2O
-
 Paenibacillus mucilaginosus TKU032 ?
-
 ?
chitosan + H2O excellent activity on colloidal chitosan (338.74%), followed by chitosan powder (185.38%) Paenibacillus mucilaginosus ?
-
 ?
chitosan + H2O excellent activity on colloidal chitosan (338.74%), followed by chitosan powder (185.38%) Paenibacillus mucilaginosus TKU032 ?
-
 ?
additional information no activity with 4-nitrophenyl-N-acetyl-beta-D-glucosaminide Paenibacillus mucilaginosus ?
-
 ?
additional information no activity with 4-nitrophenyl-N-acetyl-beta-D-glucosaminide Paenibacillus mucilaginosus TKU032 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 59000, SDS-PAGE Paenibacillus mucilaginosus

Synonyms

Synonyms Comment Organism
TKU032 chitosanase
-
 Paenibacillus mucilaginosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Paenibacillus mucilaginosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 100 50°C: about 50% of maximal activity, 100°C: about 40% of maximal activity Paenibacillus mucilaginosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Paenibacillus mucilaginosus

pH Range

pH Minimum pH Maximum Comment Organism
4 9 pH 4.0: about 75% of maximal activity, pH 9.0: about 50% of maximal activity Paenibacillus mucilaginosus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 30 min, below pH 4 activity disappears completely Paenibacillus mucilaginosus
4 8 30 min, over 80% of activity is retained Paenibacillus mucilaginosus
9
-
 30 min, 50% of the activity is retained Paenibacillus mucilaginosus