Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AlCl3 | 1 mM, 91% loss of activity | Pseudoalteromonas sp. SY39 | |
CoCl2 | 1 mM, 28% loss of activity | Pseudoalteromonas sp. SY39 | |
CuSO4 | 1 mM, 86% loss of activity | Pseudoalteromonas sp. SY39 | |
EDTA | 1 mM, 35% loss of activity | Pseudoalteromonas sp. SY39 | |
FeCl3 | 1 mM, 90% loss of activity | Pseudoalteromonas sp. SY39 | |
FeSO4 | 1 mM, 30% loss of activity | Pseudoalteromonas sp. SY39 | |
additional information | ethanol, isopropanol and dimethyl sulfoxide (5 mM -100 mM) have no effect in activity | Pseudoalteromonas sp. SY39 | |
NiCl2 | 1 mM, 26% loss of activity | Pseudoalteromonas sp. SY39 | |
SDS | 1 mM, 46% loss of activity | Pseudoalteromonas sp. SY39 | |
ZnCl2 | 1 mM, 64% loss of activity | Pseudoalteromonas sp. SY39 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 1 mM, 1.2fold activation | Pseudoalteromonas sp. SY39 | |
KCl | 1 mM, 1.2fold activation | Pseudoalteromonas sp. SY39 | |
MgSO4 | 1 mM, 1.1fold activation | Pseudoalteromonas sp. SY39 | |
NaCl | addition of 0.001-0.5M NaCl increases the CsnM activity to 128% to 151% | Pseudoalteromonas sp. SY39 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
SDS-PAGE | Pseudoalteromonas sp. SY39 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudoalteromonas sp. SY39 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudoalteromonas sp. SY39 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitosan + H2O | chitodisaccharide is the main product (69.9% of the total product) | Pseudoalteromonas sp. SY39 | chitobiose + chitooligosaccharides | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28000, SDS-PAGE | Pseudoalteromonas sp. SY39 |
Synonyms | Comment | Organism |
---|---|---|
CsnM | - |
Pseudoalteromonas sp. SY39 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Pseudoalteromonas sp. SY39 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 50 | 15°C: 49% of maximal activity, 45°C: 96% of maximal activity, 50°C: about 45% of maximal activity | Pseudoalteromonas sp. SY39 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
1 h, enzyme retains 25.4% of its initial activity | Pseudoalteromonas sp. SY39 |
40 | - |
1 h, enzyme retains 15.8% of its initial activity | Pseudoalteromonas sp. SY39 |
100 | - |
the enzyme recovers 95.2%, 89.1% and 88.1% of its initial activity after boiling for 5, 10 and 20 min, respectively | Pseudoalteromonas sp. SY39 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.9 | - |
sodium acetate buffer | Pseudoalteromonas sp. SY39 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 6.3 | pH 5.0: about 70% of maximal activity, pH 6.3: about 60% of maximal activity | Pseudoalteromonas sp. SY39 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.8 | 7.9 | the enzyme retains over 80% of its original activity after incubation at pH ranging from 5.83 to 7.92 at 4°C for 24 h | Pseudoalteromonas sp. SY39 |