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Literature summary for 3.2.1.133 extracted from

  • Kim, J.S.; Cha, S.S.; Kim, H.J.; Kim, T.J.; Ha, N.C.; Oh, S.T.; Cho, H.S.; Cho, M.J.; Kim, M.J.; Lee, H.S.; Kim, J.W.; Choi, K.Y.; Park, K.H.; Oh, B.H.
    Crystal structure of a maltogenic amylase provides insights into a catalytic versatility (1999), J. Biol. Chem., 274, 26279-26286.
    View publication on PubMed
Search for more literature for 3.2.1.133

Application

Application Comment Organism
biotechnology natural design of the transglycosylation activtiy of the enzyme at high concentrations of acceptor sugar molecules may now be altered for biotechnological applications to produce branched oligosaccharides with higher efficiency Thermus sp.

Cloned(Commentary)

Cloned (Comment) Organism
-
 Thermus sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Geobacillus stearothermophilus
crystals obtained by vapor diffusion, space group P6(1), cell dimensions of a = b = 118.04 A, c = 266.88 A Thermus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
starch + H2O Geobacillus stearothermophilus
-
 alpha-maltose + ?
-
 ?
starch + H2O Thermus sp. catalyzes the hydrolysis of starch material, central role in carbohydrate metabolism alpha-maltose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-
Thermus sp.
-
 atomic coordinates and structure factors, PDB: 1SMA
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acarbose + H2O
-
 Geobacillus stearothermophilus acarviosine-glucose + alpha-D-glucose
-
 ?
acarbose + H2O
-
 Thermus sp. acarviosine-glucose + alpha-D-glucose
-
 ?
beta-cyclodextrin + H2O
-
 Geobacillus stearothermophilus alpha-maltose + alpha-D-glucose
-
 ?
beta-cyclodextrin + H2O prefers cyclodextrins to starch or pullulan as substrate Thermus sp. alpha-maltose + alpha-D-glucose
-
 ?
additional information exhibits dual activity of alpha-D-(1,4)- and alpha-D-(1,6)- glycosidic bond cleavages, shows activity of alpha-D(1,4)- to alpha-D-(1,3), alpha-D-(1,4), or alpha-D-(1,6)-transglycosylation and cleaves acarbose, a pseudotetrasaccharide competitive inhibitor of alpha-amylases Thermus sp. ?
-
 ?
starch + H2O
-
 Geobacillus stearothermophilus alpha-maltose + ?
-
 ?
starch + H2O
-
 Thermus sp. alpha-maltose + ?
-
 ?
starch + H2O catalyzes the hydrolysis of starch material, central role in carbohydrate metabolism Thermus sp. alpha-maltose + ?
-
 ?

Subunits

Subunits Comment Organism
homodimer
-
 Thermus sp.

Synonyms

Synonyms Comment Organism
BSMA
-
 Geobacillus stearothermophilus
ThMA
-
 Thermus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Geobacillus stearothermophilus
60
-
-
 Thermus sp.