Cloned (Comment) | Organism |
---|---|
wild-type and mutant ThMAs expressed in Escherichia coli | Thermus sp. |
Protein Variants | Comment | Organism |
---|---|---|
E332D | site-directed mutagenesis, significantly decreased transglycosylation activity | Thermus sp. |
E332H | site-directed mutagenesis, replacing Glu 332 with histidine reduces transglycosylation activity significantly, but enhances hydrolysis activity on alpha-(1,3)-, alpha-(1,4)- and alpha-(1,6) glycosidic bonds relative to the wild-type | Thermus sp. |
E332Q | site-directed mutagenesis | Thermus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.426 | - |
acarbose | pH 6.0, 60°C, hydrolysis, wild-type | Thermus sp. | |
0.545 | - |
acarbose | pH 6.0, 60°C, hydrolysis, mutant E332D | Thermus sp. | |
1 | - |
acarbose | pH 6.0, 60°C, hydrolysis, mutant E332Q | Thermus sp. | |
1.08 | - |
acarbose | pH 6.0, 60°C, hydrolysis, mutant E332H | Thermus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | Thermus sp. | - |
alpha-maltose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzymes | Thermus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acarbose + alpha-D-glucose | transglycosylation | Thermus sp. | isoacarbose | - |
? | |
acarbose + H2O | hydrolysis | Thermus sp. | acarviosine-glucose + alpha-D-glucose | - |
? | |
beta-cyclodextrin + H2O | - |
Thermus sp. | alpha-maltose + alpha-D-glucose | - |
? | |
pullulan + H2O | - |
Thermus sp. | panose + ? | - |
? | |
starch + H2O | - |
Thermus sp. | alpha-maltose + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ThMA | - |
Thermus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18.7 | - |
acarbose | pH 6.0, 60°C, hydrolysis, mutant E332H | Thermus sp. | |
41.4 | - |
acarbose | pH 6.0, 60°C, hydrolysis, mutant E332Q | Thermus sp. | |
45.4 | - |
acarbose | pH 6.0, 60°C, hydrolysis, mutant E332D | Thermus sp. | |
67.8 | - |
acarbose | pH 6.0, 60°C, hydrolysis, wild-type | Thermus sp. |