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Literature summary for 3.2.1.133 extracted from

  • Kim, T.J.; Park, C.S.; Cho, H.Y.; Cha, S.S.; Kim, J.S.; Lee, S.B.; Moon, T.W.; Kim, J.W.; Oh, B.H.; Park, K.H.
    Role of the glutamate 332 residue in the transglycosylation activity of ThermusMaltogenic amylase (2000), Biochemistry, 39, 6773-6780.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant ThMAs expressed in Escherichia coli Thermus sp.

Protein Variants

Protein Variants Comment Organism
E332D site-directed mutagenesis, significantly decreased transglycosylation activity Thermus sp.
E332H site-directed mutagenesis, replacing Glu 332 with histidine reduces transglycosylation activity significantly, but enhances hydrolysis activity on alpha-(1,3)-, alpha-(1,4)- and alpha-(1,6) glycosidic bonds relative to the wild-type Thermus sp.
E332Q site-directed mutagenesis Thermus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.426
-
acarbose pH 6.0, 60°C, hydrolysis, wild-type Thermus sp.
0.545
-
acarbose pH 6.0, 60°C, hydrolysis, mutant E332D Thermus sp.
1
-
acarbose pH 6.0, 60°C, hydrolysis, mutant E332Q Thermus sp.
1.08
-
acarbose pH 6.0, 60°C, hydrolysis, mutant E332H Thermus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
starch + H2O Thermus sp.
-
alpha-maltose + ?
-
?

Organism

Organism UniProt Comment Textmining
Thermus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzymes Thermus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acarbose + alpha-D-glucose transglycosylation Thermus sp. isoacarbose
-
?
acarbose + H2O hydrolysis Thermus sp. acarviosine-glucose + alpha-D-glucose
-
?
beta-cyclodextrin + H2O
-
Thermus sp. alpha-maltose + alpha-D-glucose
-
?
pullulan + H2O
-
Thermus sp. panose + ?
-
?
starch + H2O
-
Thermus sp. alpha-maltose + ?
-
?

Synonyms

Synonyms Comment Organism
ThMA
-
Thermus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
18.7
-
acarbose pH 6.0, 60°C, hydrolysis, mutant E332H Thermus sp.
41.4
-
acarbose pH 6.0, 60°C, hydrolysis, mutant E332Q Thermus sp.
45.4
-
acarbose pH 6.0, 60°C, hydrolysis, mutant E332D Thermus sp.
67.8
-
acarbose pH 6.0, 60°C, hydrolysis, wild-type Thermus sp.