Cloned (Comment) | Organism |
---|---|
cloned and overproduced in Escherichia coli MC1061 | Thermus sp. |
Crystallization (Comment) | Organism |
---|---|
mutant E357L, space group P6(1), a = b = 119.09, c = 270.20 | Thermus sp. |
Protein Variants | Comment | Organism |
---|---|---|
E357L | site-directed mutagenesis | Thermus sp. |
W47A | site-directed mutagenesis | Thermus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km 131 mg/ml, starch as substrate, mutant W47A | Thermus sp. | |
additional information | - |
additional information | Km 73.5 mg/ml, starch as substrate, wild-type | Thermus sp. | |
0.174 | - |
beta-cyclodextrin | pH 6.0, 60°C, wild-type | Thermus sp. | |
2.03 | - |
beta-cyclodextrin | pH 6.0, 60°C, mutant W47A | Thermus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
mutant E357L | Thermus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclomaltodextrin + H2O | - |
Thermus sp. | alpha-maltose + alpha-D-glucose | - |
? | |
additional information | nearly indistinguishable from cyclomaltodextrinase from Bacillus sp. and Bacillus stearothermophilus neopullulanase, distinguished from typicsl alpha-amylases by containing a novel N-terminal domain and exhibiting preferential substrate specificities for cyclomaltodextrins over starch | Thermus sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Thermus sp. |
Synonyms | Comment | Organism |
---|---|---|
ThMA | - |
Thermus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
126 | - |
beta-cyclodextrin | - |
Thermus sp. | |
180 | - |
beta-cyclodextrin | pH 6.0, 60°C, mutantW47A | Thermus sp. | |
249 | - |
starch | pH 6.0, 60°C, wild-type | Thermus sp. | |
335 | - |
starch | pH 6.0, 60°C, mutant W47A | Thermus sp. |