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Literature summary for 3.2.1.133 extracted from

  • Lee, H.S.; Kim, M.S.; Cho, H.S.; Kim, J.I.; Kim, T.J.; Choi, J.H.; Park, C.; Oh, B.H.; Park, K.H.
    Cyclomaltodextrinase, neopullulanase, and maltogenic amylase are nearly indistinguishable from each other (2002), J. Biol. Chem., 277, 21891-21897.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloned and overproduced in Escherichia coli MC1061 Thermus sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant E357L, space group P6(1), a = b = 119.09, c = 270.20 Thermus sp.

Protein Variants

Protein Variants Comment Organism
E357L site-directed mutagenesis Thermus sp.
W47A site-directed mutagenesis Thermus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km 131 mg/ml, starch as substrate, mutant W47A Thermus sp.
additional information
-
additional information Km 73.5 mg/ml, starch as substrate, wild-type Thermus sp.
0.174
-
beta-cyclodextrin pH 6.0, 60°C, wild-type Thermus sp.
2.03
-
beta-cyclodextrin pH 6.0, 60°C, mutant W47A Thermus sp.

Organism

Organism UniProt Comment Textmining
Thermus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
mutant E357L Thermus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclomaltodextrin + H2O
-
Thermus sp. alpha-maltose + alpha-D-glucose
-
?
additional information nearly indistinguishable from cyclomaltodextrinase from Bacillus sp. and Bacillus stearothermophilus neopullulanase, distinguished from typicsl alpha-amylases by containing a novel N-terminal domain and exhibiting preferential substrate specificities for cyclomaltodextrins over starch Thermus sp. ?
-
?

Subunits

Subunits Comment Organism
dimer
-
Thermus sp.

Synonyms

Synonyms Comment Organism
ThMA
-
Thermus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
126
-
beta-cyclodextrin
-
Thermus sp.
180
-
beta-cyclodextrin pH 6.0, 60°C, mutantW47A Thermus sp.
249
-
starch pH 6.0, 60°C, wild-type Thermus sp.
335
-
starch pH 6.0, 60°C, mutant W47A Thermus sp.