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Literature summary for 3.2.1.133 extracted from
- Molecular cloning and biochemical characterization of the first archaeal maltogenic amylase from the hyperthermophilic archaeon Thermoplasma volcanium GSS1 (2007), Biochim. Biophys. Acta, 1774, 661-669.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli MC1061 | Thermoplasma volcanium |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.5 | - |
maltoheptaose | kinetic parameters for C14-labelled maltooligosaccharides | Thermoplasma volcanium |  |
| 1.6 | - |
maltotetraose | kinetic parameters for C14-labelled maltooligosaccharides | Thermoplasma volcanium | |
| 3.6 | - |
maltotriose | kinetic parameters for C14-labelled maltooligosaccharides | Thermoplasma volcanium | |
| 3.8 | - |
maltohexaose | kinetic parameters for C14-labelled maltooligosaccharides | Thermoplasma volcanium | |
| 4.1 | - |
soluble starch | estimated for monomeric conformation, in presence of 1 M NaCl | Thermoplasma volcanium | |
| 4.4 | - |
maltopentaose | kinetic parameters for C14-labelled maltooligosaccharides | Thermoplasma volcanium | |
| 13 | - |
soluble starch | estimated for the high oligomer conformation | Thermoplasma volcanium |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72060 | - |
MALDI-TOF | Thermoplasma volcanium |
| 72090 | - |
deduced from sequence | Thermoplasma volcanium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | Thermoplasma volcanium | transglycosylation pattern opposite to that of bacterial maltogenic amylases, predominant formation of alpha-1,4-glycosidic linked transfer products than of alpha-1,6-linked products | alpha-maltose + ? | - |
? | |
| starch + H2O | Thermoplasma volcanium GSS1 | transglycosylation pattern opposite to that of bacterial maltogenic amylases, predominant formation of alpha-1,4-glycosidic linked transfer products than of alpha-1,6-linked products | alpha-maltose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoplasma volcanium | - |
GSS1 | - |
| Thermoplasma volcanium GSS1 | - |
GSS1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein, gel filtration | Thermoplasma volcanium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
specific activity of starch hydrolysis estimated for the high oligomer conformation is about 7 U/mg and for monomeric state about 110 U/mg | Thermoplasma volcanium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acarbose + H2O | - |
Thermoplasma volcanium | alpha-maltose + ? | - |
? | |
| acarbose + H2O | - |
Thermoplasma volcanium GSS1 | alpha-maltose + ? | - |
? | |
| beta-cyclodextrin + H2O | high thermostability, substrate preference dependent on oligomeric state | Thermoplasma volcanium | alpha-maltose + alpha-D-glucose | - |
? | |
| beta-cyclodextrin + H2O | high thermostability, substrate preference dependent on oligomeric state | Thermoplasma volcanium GSS1 | alpha-maltose + alpha-D-glucose | - |
? | |
| maltoheptaose + H2O | - |
Thermoplasma volcanium | maltose + ? | - |
? | |
| maltohexaose + H2O | - |
Thermoplasma volcanium | maltose + ? | - |
? | |
| maltopentaose + H2O | - |
Thermoplasma volcanium | alpha-maltose + ? | - |
? | |
| maltotetraose + H2O | - |
Thermoplasma volcanium | alpha-maltose + ? | - |
? | |
| maltotriose + H2O | - |
Thermoplasma volcanium | alpha-maltose + alpha-D-glucose | - |
? | |
| maltotriose + H2O | - |
Thermoplasma volcanium GSS1 | alpha-maltose + alpha-D-glucose | - |
? | |
| pullulan + H2O | - |
Thermoplasma volcanium | alpha-maltose + ? | - |
? | |
| soluble starch + H2O | - |
Thermoplasma volcanium | alpha-maltose + ? | - |
? | |
| starch + H2O | - |
Thermoplasma volcanium | alpha-maltose + ? | - |
? | |
| starch + H2O | transglycosylation pattern opposite to that of bacterial maltogenic amylases, predominant formation of alpha-1,4-glycosidic linked transfer products than of alpha-1,6-linked products | Thermoplasma volcanium | alpha-maltose + ? | - |
? | |
| starch + H2O | - |
Thermoplasma volcanium GSS1 | alpha-maltose + ? | - |
? | |
| starch + H2O | transglycosylation pattern opposite to that of bacterial maltogenic amylases, predominant formation of alpha-1,4-glycosidic linked transfer products than of alpha-1,6-linked products | Thermoplasma volcanium GSS1 | alpha-maltose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | recombinant protein, high oligomer in solution, dissociation into dimer and monomer mixture by a high concentration of NaCl | Thermoplasma volcanium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glucan 1,4-alpha-maltohydrolase | - |
Thermoplasma volcanium |
| maltogenic amylase | - |
Thermoplasma volcanium |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | 80 | high thermostability, dependent on oligomer structure of the enzyme, optimal activity at 75°C and 80°C for beta-cyclodextrin and soluble starch, half-lifes of 61 min at 75°C and of 24 min at 80°C | Thermoplasma volcanium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
high thermostability, half-lives of thermal inactivation and melting temperature tested | Thermoplasma volcanium |
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