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Literature summary for 3.2.1.133 extracted from
- Extracellular secretion of a maltogenic amylase from Lactobacillus gasseri ATCC33323 in Lactococcus lactis MG1363 and its application on the production of branched maltooligosaccharides (2007), J. Microbiol. Biotechnol., 17, 1521-1526.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | production of branched maltooligosaccharide | Lactobacillus gasseri |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| amplified in Escherichia coli DH5alpha, heterogenous expression in Lactococcus lactis MG1363, P170 expression system for production of recombinant LGMA | Lactobacillus gasseri |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | secretion | Lactobacillus gasseri | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
SDS-PAGE, difference in molecular masses due to the presence of the six-histidine tag and two additional amino acids | Lactobacillus gasseri |
| 66630 | - |
deduced from sequence | Lactobacillus gasseri |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | Lactobacillus gasseri | - |
alpha-maltose + ? | - |
? |  |
| starch + H2O | Lactobacillus gasseri ATCC 33323 | - |
alpha-maltose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactobacillus gasseri | - |
ATCC33323 | - |
| Lactobacillus gasseri ATCC 33323 | - |
ATCC33323 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| His-tagged recombinant protein purified via Ni-NTA colummns | Lactobacillus gasseri |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture fluid | activity tested in both, liquid and solid media | Lactobacillus gasseri | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
catalytic activity of wild-type LGMA and recombinant rLGMA tested in liquid and solid media, kinetic properties determined by amount of reducing sugars, assayed according to the dinitrosalicylic acid method, reaction at pH 6.5 for 13.5 h at 50°C, yields of 53.1% obtained | Lactobacillus gasseri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-cyclodextrin + H2O | recombinant rLGMA, expressed in Escherichia coli and in Lactococcus lactis MG1363 | Lactobacillus gasseri | alpha-maltose + ? | - |
? | |
| beta-cyclodextrin + H2O | recombinant rLGMA, expressed in Escherichia coli and in Lactococcus lactis MG1363 | Lactobacillus gasseri ATCC 33323 | alpha-maltose + ? | - |
? | |
| maltotriose + H2O | recombinant rLGMA, expressed in Escherichia coli and in Lactococcus lactis MG1363 | Lactobacillus gasseri | ? | - |
? | |
| maltotriose + H2O | recombinant rLGMA, expressed in Escherichia coli and in Lactococcus lactis MG1363 | Lactobacillus gasseri ATCC 33323 | ? | - |
? | |
| pullulan + H2O | recombinant rLGMA, expressed in Escherichia coli and in Lactococcus lactis MG1363 | Lactobacillus gasseri | alpha-maltose + ? | - |
? | |
| pullulan + H2O | recombinant rLGMA, expressed in Escherichia coli and in Lactococcus lactis MG1363 | Lactobacillus gasseri ATCC 33323 | alpha-maltose + ? | - |
? | |
| starch + H2O | - |
Lactobacillus gasseri | alpha-maltose + ? | - |
? | |
| starch + H2O | wild-type LGMA and recombinant rLGMA, reaction products determined by thin-layer chromatography and gel filtration | Lactobacillus gasseri | alpha-maltose + ? | - |
? | |
| starch + H2O | - |
Lactobacillus gasseri ATCC 33323 | alpha-maltose + ? | - |
? | |
| starch + H2O | wild-type LGMA and recombinant rLGMA, reaction products determined by thin-layer chromatography and gel filtration | Lactobacillus gasseri ATCC 33323 | alpha-maltose + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glucan 1,4-alpha-maltohydrolase | - |
Lactobacillus gasseri |
| LGMA | - |
Lactobacillus gasseri |
| maltogenic amylase | - |
Lactobacillus gasseri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
recombinant rLGMA | Lactobacillus gasseri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
recombinant rLGMA | Lactobacillus gasseri |
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