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Literature summary for 3.2.1.133 extracted from

  • Li, X.; Li, D.; Yin, Y.; Park, K.H.
    Characterization of a recombinant amylolytic enzyme of hyperthermophilic archaeon Thermofilum pendens with extremely thermostable maltogenic amylase activity (2010), Appl. Microbiol. Biotechnol., 85, 1821-1830.
    View publication on PubMed
Search for more literature for 3.2.1.133

Activating Compound

Activating Compound Comment Organism Structure
DMSO enzyme activity increases to 136% in the presence of 10% (v/v) DMSO Thermofilum pendens
ethanol enzyme activity increases to 113% in the presence of 10% (v/v) ethanol Thermofilum pendens
methanol enzyme activity increases to 104.4% in the presence of 10% (v/v) methanol Thermofilum pendens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Thermofilum pendens

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ enzyme activity decreases to 77.3% in the presence of 5 mM BaCl2 Thermofilum pendens
Cu2+ enzyme activity decreases to 17% in the presence of 5 mM CuCl2 Thermofilum pendens
Fe2+ enzyme activity decreases to 18.4% in the presence of 5 mM FeSO4 Thermofilum pendens
Hg2+ enzyme activity decreases to 17.5% in the presence of 5 mM HgCl2 Thermofilum pendens
Mn2+ enzyme activity decreases to 50.2% in the presence of 5 mM MnCl2 Thermofilum pendens
Zn2+ enzyme activity decreases to 17.8% in the presence of 5 mM ZnCl2 Thermofilum pendens

Metals/Ions

Metals/Ions Comment Organism Structure
Al3+ enzyme activity increases to 146.8% in the presence of 5 mM AlCl3 Thermofilum pendens
Ca2+ enzyme activity increases to 136% in the presence of 5 mM CaCl2 Thermofilum pendens
Mg2+ enzyme activity increases to 130% in the presence of 5 mM CaCl2 Thermofilum pendens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
 x * 74000, SDS-PAGE Thermofilum pendens
74252
-
 x * 74252, calculated from amino acid sequence Thermofilum pendens

Organic Solvent Stability

Organic Solvent Comment Organism
DMSO enzyme activity increases to 136% in the presence of 10% (v/v) DMSO Thermofilum pendens
Ethanol enzyme activity increases to 113% in the presence of 10% (v/v) ethanol Thermofilum pendens
Methanol enzyme activity increases to 104.4% in the presence of 10% (v/v) methanol Thermofilum pendens

Organism

Organism UniProt Comment Textmining
Thermofilum pendens
-
-
-
Thermofilum pendens Hrk 5 (DSM 2475)
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Thermofilum pendens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
91
-
 after 3fold purification, using gamma-cyclodextrin as substrate, at 90°C in 50 mM sodium acetate buffer (pH 5.5), with 5 mM CuCl2 Thermofilum pendens
93.6
-
 after 3fold purification, using gamma-cyclodextrin as substrate, at 90°C in 50 mM sodium acetate buffer (pH 5.5), with 5 mM HgCl2 Thermofilum pendens
95.2
-
 after 3fold purification, using gamma-cyclodextrin as substrate, at 90°C in 50 mM sodium acetate buffer (pH 5.5), with 5 mM ZnCl2 Thermofilum pendens
98.5
-
 after 3fold purification, using gamma-cyclodextrin as substrate, at 90°C in 50 mM sodium acetate buffer (pH 5.5), with 5 mM FeSO4 Thermofilum pendens
177.4
-
 cell extract, using gamma-cyclodextrin as substrate, at 90°C in 50 mM sodium acetate buffer (pH 5.5) Thermofilum pendens
268.6
-
 after 3fold purification, using gamma-cyclodextrin as substrate, at 90°C in 50 mM sodium acetate buffer (pH 5.5), with 5 mM MnCl2 Thermofilum pendens
413.6
-
 after 3fold purification, using gamma-cyclodextrin as substrate, at 90°C in 50 mM sodium acetate buffer (pH 5.5), with 5 mM BaCl2 Thermofilum pendens
535.1
-
 after 3fold purification, using gamma-cyclodextrin as substrate, at 90°C in 50 mM sodium acetate buffer (pH 5.5) Thermofilum pendens
558.6
-
 after 3fold purification, using gamma-cyclodextrin as substrate, at 90°C in 50 mM sodium acetate buffer (pH 5.5), with 10% (v/v) methanol Thermofilum pendens
568.8
-
 after 3fold purification, using gamma-cyclodextrin as substrate, at 90°C in 50 mM sodium acetate buffer (pH 5.5), with 5 mM CoCl2 Thermofilum pendens
592.9
-
 after 3fold purification, using gamma-cyclodextrin as substrate, at 90°C in 50 mM sodium acetate buffer (pH 5.5), with 10% (v/v) DMSO Thermofilum pendens
604.7
-
 after 3fold purification, using gamma-cyclodextrin as substrate, at 90°C in 50 mM sodium acetate buffer (pH 5.5), with 10% (v/v) ethanol Thermofilum pendens
695.6
-
 after 3fold purification, using gamma-cyclodextrin as substrate, at 90°C in 50 mM sodium acetate buffer (pH 5.5), with 5 mM MgCl2 Thermofilum pendens
728.8
-
 after 3fold purification, using gamma-cyclodextrin as substrate, at 90°C in 50 mM sodium acetate buffer (pH 5.5), with 5 mM CaCl2 Thermofilum pendens
785.5
-
 after 3fold purification, using gamma-cyclodextrin as substrate, at 90°C in 50 mM sodium acetate buffer (pH 5.5), with 5 mM AlCl3 Thermofilum pendens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acarbose + H2O
-
 Thermofilum pendens acarviosine-glucose + D-glucose
-
 ?
alpha-cyclodextrin + H2O 64.7% activity compared to gamma-cyclodextrin Thermofilum pendens ?
-
 ?
beta-cyclodextrin + H2O 78.1% activity compared to gamma-cyclodextrin Thermofilum pendens ?
-
 ?
gamma-cyclodextrin + H2O maximal activity (100%) Thermofilum pendens alpha-maltose + alpha-D-glucose
-
 ?
additional information maltooligosaccharides G3-G7 show 5.4-24.1% relative activity compared to gamma-cyclodextrin Thermofilum pendens ?
-
 ?
pullulan + H2O
-
 Thermofilum pendens panose + ?
-
 ?
starch + H2O
-
 Thermofilum pendens alpha-maltose + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 74000, SDS-PAGE Thermofilum pendens
? x * 74252, calculated from amino acid sequence Thermofilum pendens

Synonyms

Synonyms Comment Organism
maltogenic amylase
-
 Thermofilum pendens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
-
 Thermofilum pendens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
80 105 at 105°C, almost 50% of the maximum activity is detected Thermofilum pendens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85 100 purified enzyme is extremely thermostable with a half-life of 60 min at an optimal temperature of 95°C. The enzyme retains about 80% relative activity after 60 min of incubation at 85°C and 90°C, about 50% relative activity after 60 min of incubation at 95°C, and about 25% relative activity after 60 min of incubation at 100°C Thermofilum pendens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Thermofilum pendens

pH Range

pH Minimum pH Maximum Comment Organism
5 6.5 more than 50% of the maximum activity is retained in the range between pH 5.0 and pH 6.5 Thermofilum pendens