Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli MC1061 cells | Staphylothermus marinus |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 12% (w/v) polyethylene glycol 4000, 2% (v/v) isopropyl alcohol, 0.1 M ADA, pH 6.5, and 0.1 M Li2SO4 | Staphylothermus marinus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
alpha-cyclodextrin | pH and temperature not specified in the publication | Staphylothermus marinus | |
1.48 | - |
beta-cyclodextrin | pH and temperature not specified in the publication | Staphylothermus marinus | |
2.68 | - |
gamma-cyclodextrin | pH and temperature not specified in the publication | Staphylothermus marinus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylothermus marinus | A3DM60 | - |
- |
Staphylothermus marinus DSM 3639 | A3DM60 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Staphylothermus marinus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-cyclodextrin + H2O | - |
Staphylothermus marinus | ? | - |
? | |
alpha-cyclodextrin + H2O | - |
Staphylothermus marinus DSM 3639 | ? | - |
? | |
amylopectin + H2O | - |
Staphylothermus marinus | maltose + D-glucose | - |
? | |
amylopectin + H2O | - |
Staphylothermus marinus DSM 3639 | maltose + D-glucose | - |
? | |
amylose + H2O | - |
Staphylothermus marinus | maltose + D-glucose | - |
? | |
amylose + H2O | - |
Staphylothermus marinus DSM 3639 | maltose + D-glucose | - |
? | |
beta-cyclodextrin + H2O | - |
Staphylothermus marinus | ? | - |
? | |
beta-cyclodextrin + H2O | - |
Staphylothermus marinus DSM 3639 | ? | - |
? | |
gamma-cyclodextrin + H2O | - |
Staphylothermus marinus | ? | - |
? | |
gamma-cyclodextrin + H2O | - |
Staphylothermus marinus DSM 3639 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
maltogenic amylase | - |
Staphylothermus marinus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
- |
Staphylothermus marinus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
109 | - |
109°C melting temperature with enzymatic activity under acidic conditions (pH 3.5-5.0) | Staphylothermus marinus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.59 | - |
amylose | pH and temperature not specified in the publication | Staphylothermus marinus | |
0.8 | - |
amylopectin | pH and temperature not specified in the publication | Staphylothermus marinus | |
1.45 | - |
alpha-cyclodextrin | pH and temperature not specified in the publication | Staphylothermus marinus | |
5.36 | - |
beta-cyclodextrin | pH and temperature not specified in the publication | Staphylothermus marinus | |
29.1 | - |
gamma-cyclodextrin | pH and temperature not specified in the publication | Staphylothermus marinus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.37 | - |
alpha-cyclodextrin | pH and temperature not specified in the publication | Staphylothermus marinus | |
3.62 | - |
beta-cyclodextrin | pH and temperature not specified in the publication | Staphylothermus marinus | |
10.86 | - |
gamma-cyclodextrin | pH and temperature not specified in the publication | Staphylothermus marinus |