Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5alpha cells | Bacillus sp. (in: Bacteria) |
Protein Variants | Comment | Organism |
---|---|---|
D46G | the mutant behaves similar to the wild type enzyme regarding thermoactivity, thermostability and pH profile. The Km values of the mutant for all substrates are strongly increased compared to the wild type enzyme | Bacillus sp. (in: Bacteria) |
D46N | the mutant behaves similar to the wild type enzyme regarding kinetic parameters, thermoactivity, thermostability and pH profile | Bacillus sp. (in: Bacteria) |
D46V | the mutant behaves similar to the wild type enzyme regarding thermoactivity, thermostability and pH profile. The affinity and catalytic efficiency of the mutant toward beta-cyclodextrin are increased 5fold as compared with the wild type enzyme | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67500 | - |
x * 67500, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | A7DWA8 | - |
- |
Bacillus sp. (in: Bacteria) US149 | A7DWA8 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and fast-performance liquid chromatography | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-cyclodextrin + H2O | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
beta-cyclodextrin + H2O | - |
Bacillus sp. (in: Bacteria) US149 | ? | - |
? | |
potato starch + H2O | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
potato starch + H2O | - |
Bacillus sp. (in: Bacteria) US149 | ? | - |
? | |
pullulan + H2O | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
pullulan + H2O | - |
Bacillus sp. (in: Bacteria) US149 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 67500, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
MAase | - |
Bacillus sp. (in: Bacteria) |
maltogenic amylase | - |
Bacillus sp. (in: Bacteria) |
MAUS149 | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
half-life at 55°C is about 15 min | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Bacillus sp. (in: Bacteria) |