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Literature summary for 3.2.1.139 extracted from
- Isolation and analysis of a gene encoding alpha-glucuronidase, an enzyme with a novel primary structure involved in the breakdown of xylan (1997), Mol. Microbiol., 23, 267-279.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Thermotoga maritima |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Thermotoga maritima |  |
| Cd2+ | 1 mM, strong inhibition | Thermotoga maritima | |
| Cu2+ | 1 mM, strong inhibition | Thermotoga maritima | |
| Hg2+ | 1 mM, strong inhibition | Thermotoga maritima | |
| p-hydroxymercuribenzoate | - |
Thermotoga maritima | |
| Pb2+ | 1 mM, strong inhibition | Thermotoga maritima | |
| Zn2+ | 1 mM, strong inhibition | Thermotoga maritima | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.95 | - |
4-O-Methyl-glucuronosyl-xylobiose | - |
Thermotoga maritima | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | - |
- |
- |
| Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-O-methyl-glucuronosyl-xylobiose + H2O | - |
Thermotoga maritima | 4-O-methyl-glucuronate + xylobiose | - |
? | |
| 4-O-methyl-glucuronosyl-xylobiose + H2O | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | 4-O-methyl-glucuronate + xylobiose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
- |
Thermotoga maritima |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 100 | about 45% of maximal activity at 60°C and at 100°C | Thermotoga maritima |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | 75 | partial inactivation, but thereafter the residual activity remains nearly constant for several days | Thermotoga maritima |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | - |
- |
Thermotoga maritima |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | pH 5: about 35% of maximal activity, pH 8: about 45% of maximal activity | Thermotoga maritima |
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