Literature summary for 3.2.1.139 extracted from

Zaide, G.; Shallom, D.; Shulami, S.; Zolotnitsky, G.; Golan, G.; Baasov, T.; Shoham, G.; Shoham, Y.
Biochemical characterization and identification of catalytic residues in alpha-glucuronidase from Bacillus stearothermophilus T-6 (2001), Eur. J. Biochem., 268, 3006-3016.

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Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Escherichia coli using the T7 polymerase expression system	Geobacillus stearothermophilus

Protein Variants

Protein Variants	Comment	Organism
D274A	as active as wild-type enzyme	Geobacillus stearothermophilus
D364A	0.000015% of wild-type activity	Geobacillus stearothermophilus
D364A/E392C	0.0000004% of wild-type activity	Geobacillus stearothermophilus
E158N	0.07% of wild-type activity	Geobacillus stearothermophilus
E285N	0.0002% of wild-type activity	Geobacillus stearothermophilus
E386Q	0.07% of wild-type activity	Geobacillus stearothermophilus
E392C	0.00002% of wild-type activity	Geobacillus stearothermophilus
E510A	20% of wild-type activity	Geobacillus stearothermophilus
additional information	truncated forms of the enzyme, lacking either 126 amino acids from its N-terminus or 81 amino acids from its C-terminus, exhibit low residual activity	Geobacillus stearothermophilus

Inhibitors

Inhibitors	Comment	Organism	Structure
Ag+	1 mM, 90% inhibition	Geobacillus stearothermophilus
Cu2+	0.1 mM, complete inhibition	Geobacillus stearothermophilus
Hg2+	25% inhibition at 0.1 mM, complete inhibition at 1 mM	Geobacillus stearothermophilus
K+	1 mM, 15% inhibition	Geobacillus stearothermophilus
Ni2+	1 mM, 10% inhibition	Geobacillus stearothermophilus
Zn2+	1 mM, 55% inhibition	Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.2	-	aldotetraouronic acid	55°C	Geobacillus stearothermophilus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe3+	1 mM, 25% activation	Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
78480	-	x * 78480, calculated from sequence	Geobacillus stearothermophilus

Organism

Organism	UniProt	Comment	Textmining
Geobacillus stearothermophilus	-	-	-
Geobacillus stearothermophilus T-6	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant	Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
42	-	-	Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
aldotetraouronic acid + H2O	-	Geobacillus stearothermophilus	?	-	?
aldotetraouronic acid + H2O	-	Geobacillus stearothermophilus T-6	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 78480, calculated from sequence	Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
65	-	-	Geobacillus stearothermophilus

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
45	70	45°C: about 35% of maximal activity, 70°C: about 50% of maximal activity	Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
70	-	20 min, no less of activity	Geobacillus stearothermophilus
73.4	-	denaturation at	Geobacillus stearothermophilus
75	-	20 min, 70% loss of activity	Geobacillus stearothermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
54.9	-	aldotetraouronic acid	55°C	Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	6	-	Geobacillus stearothermophilus

pH Range

pH Minimum	pH Maximum	Comment	Organism
4.5	7.5	about 40% of maximal activity at pH 4.5 and pH 7.5	Geobacillus stearothermophilus

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Release 2023.1 (January 2023)