Crystallization (Comment) | Organism |
---|---|
crystal structure of mutant E292A in complex with its substrate aldobiouronic acid | Cellvibrio japonicus |
Protein Variants | Comment | Organism |
---|---|---|
E292A | inactive mutant enzyme | Cellvibrio japonicus |
K288A | kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is 0.003% of wild-type value | Cellvibrio japonicus |
K360A | kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is 0.019% of wild-type value | Cellvibrio japonicus |
R325A | kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is 0.000096% of wild-type value | Cellvibrio japonicus |
V210A | kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is 38% of wild-type value | Cellvibrio japonicus |
V210G | kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is identical to wild-type value | Cellvibrio japonicus |
V210N | kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is 9.4% of wild-type value | Cellvibrio japonicus |
V210S | kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is 4.3% of wild-type value | Cellvibrio japonicus |
W160A | kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is 0.0064% of wild-type value | Cellvibrio japonicus |
W543A | kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is 0.069% of wild-type value | Cellvibrio japonicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-O-Methyl-D-glucuronic acid | - |
Cellvibrio japonicus | |
D-glucose | - |
Cellvibrio japonicus | |
D-glucuronic acid | - |
Cellvibrio japonicus | |
D-xylose | - |
Cellvibrio japonicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside | 37°C, pH 7.0, mutant enzyme V210G | Cellvibrio japonicus | |
0.21 | - |
4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside | 37°C, pH 7.0, wild-type enzyme | Cellvibrio japonicus | |
0.69 | - |
4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside | 37°C, pH 7.0, mutant enzyme K288A | Cellvibrio japonicus | |
0.82 | - |
4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside | 37°C, pH 7.0, mutant enzyme W543A | Cellvibrio japonicus | |
0.86 | - |
4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside | 37°C, pH 7.0, mutant enzyme V210A | Cellvibrio japonicus | |
1.6 | - |
4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside | 37°C, pH 7.0, mutant enzyme K360A | Cellvibrio japonicus | |
2.1 | - |
4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside | 37°C, pH 7.0, mutant enzyme W160A | Cellvibrio japonicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cellvibrio japonicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cellvibrio japonicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside + H2O | - |
Cellvibrio japonicus | 4-nitrophenyl-D-xylopyranoside + 4-O-methyl-alpha-D-glucuronate | - |
? | |
aldobiouronic acid + H2O | - |
Cellvibrio japonicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GlcA67A | - |
Cellvibrio japonicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside | 37°C, pH 7.0, mutant enzyme K288A | Cellvibrio japonicus | |
0.09 | - |
4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside | 37°C, pH 7.0, mutant enzyme W160A | Cellvibrio japonicus | |
0.2 | - |
4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside | 37°C, pH 7.0, mutant enzyme K360A | Cellvibrio japonicus | |
0.38 | - |
4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside | 37°C, pH 7.0, mutant enzyme W543A | Cellvibrio japonicus | |
127 | - |
4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside | 37°C, pH 7.0, mutant enzyme V210G | Cellvibrio japonicus | |
143 | - |
4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside | 37°C, pH 7.0, wild-type enzyme | Cellvibrio japonicus | |
220 | - |
4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside | 37°C, pH 7.0, mutant enzyme V210A | Cellvibrio japonicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.54 | - |
4-O-methyl-glucuronic acid | 37°C, pH 7.0, wild-type enzyme | Cellvibrio japonicus | |
3.84 | - |
glucuronic acid | 37°C, pH 7.0, wild-type enzyme | Cellvibrio japonicus | |
64.3 | - |
glucuronic acid | 37°C, pH 7.0, mutant enzyme V210N | Cellvibrio japonicus | |
69.5 | - |
xylose | 37°C, pH 7.0, wild-type enzyme | Cellvibrio japonicus | |
77.1 | - |
glucose | 37°C, pH 7.0, wild-type enzyme | Cellvibrio japonicus | |
78.3 | - |
glucuronic acid | 37°C, pH 7.0, mutant enzyme V210S | Cellvibrio japonicus |