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Literature summary for 3.2.1.14 extracted from
- Purifications and some properties of two chitinases from Streptomyces orientalis which lyse Rhizopus cell wall (1976), Agric. Biol. Chem., 40, 2325-2333.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Amycolatopsis orientalis |  |
| Fe2+ | - |
Amycolatopsis orientalis | |
| Hg2+ | - |
Amycolatopsis orientalis | |
| Pb2+ | - |
Amycolatopsis orientalis | |
| Sn2+ | - |
Amycolatopsis orientalis | |
| Zn2+ | - |
Amycolatopsis orientalis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Amycolatopsis orientalis | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
gel filtration, isoenzyme II | Amycolatopsis orientalis |
| 33000 | - |
gel filtration, isoenzyme I | Amycolatopsis orientalis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Amycolatopsis orientalis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, isoenzyme I 30fold, isoenzyme II 21fold | Amycolatopsis orientalis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Amycolatopsis orientalis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cell wall + H2O | from Rhizopus delemar | Amycolatopsis orientalis | chitin oligosaccharides | N-acetylglucosamine only | ? | |
| chitin + H2O | - |
Amycolatopsis orientalis | N,N-diacetylchitobiose + N-acetylglucosamine | N-acetylglucosamine only | ? | |
| glycol chitin + H2O | - |
Amycolatopsis orientalis | N-acetylglucosamine + ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
isoenzyme I | Amycolatopsis orientalis |
| 60 | - |
isoenzyme II | Amycolatopsis orientalis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
stable below 50°C | Amycolatopsis orientalis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6.5 | - |
Amycolatopsis orientalis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | - |
Amycolatopsis orientalis |
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