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Literature summary for 3.2.1.14 extracted from
- Purification and characteristics of an autolytic chitinase of Piromyces communis OTS1 from culture medium (1997), Curr. Microbiol., 35, 48-51.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| allosamidin | - |
Piromyces communis |  |
| EDTA | - |
Piromyces communis | |
| Hg2+ | - |
Piromyces communis | |
| phenylmethanesulfonylflouride | - |
Piromyces communis | |
| SDS | - |
Piromyces communis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Piromyces communis | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44900 | - |
x * 44900, SDS-PAGE | Piromyces communis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Piromyces communis | - |
OTS1 | - |
| Piromyces communis OTS1 | - |
OTS1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 24.06fold | Piromyces communis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Piromyces communis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
1004.33 pkat/mg | Piromyces communis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-N,N'N''-triacetyl-beta-chitotrioside + H2O | - |
Piromyces communis | ? + 4-methylumbelliferone | - |
? | |
| 4-methylumbelliferyl-N,N'N''-triacetyl-beta-chitotrioside + H2O | - |
Piromyces communis OTS1 | ? + 4-methylumbelliferone | - |
? | |
| cell wall + H2O | fungal cell wall | Piromyces communis | chitin oligosaccharides | - |
? | |
| cell wall + H2O | fungal cell wall | Piromyces communis OTS1 | chitin oligosaccharides | - |
? | |
| chitin + H2O | colloidal chitin: trace amounts of product, regenerated chitin: slowly, powdered chitin: rapidly | Piromyces communis | N,N-diacetylchitobiose + N-acetylglucosamine | - |
? | |
| chitin + H2O | colloidal chitin: trace amounts of product, regenerated chitin: slowly, powdered chitin: rapidly | Piromyces communis OTS1 | N,N-diacetylchitobiose + N-acetylglucosamine | - |
? | |
| glycol chitin + H2O | slowly | Piromyces communis | N-acetylglucosamine + ? | - |
? | |
| glycol chitin + H2O | slowly | Piromyces communis OTS1 | N-acetylglucosamine + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 44900, SDS-PAGE | Piromyces communis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 60 | 50% of activity remains between 40 and 60°C | Piromyces communis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Piromyces communis |
| 70 | - |
enzyme inactivated at 70°C | Piromyces communis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | - |
Piromyces communis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | - |
Piromyces communis |
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