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Literature summary for 3.2.1.14 extracted from

  • Kim, K.J.; Yang, Y.J.; Kim, J.G.
    Purification and characterization of chitinase from Streptomyces sp. M-20 (2003), J. Biochem. Mol. Biol., 36, 185-189.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol
-
Streptomyces sp.

Inhibitors

Inhibitors Comment Organism Structure
Ag+ strong inhibition Streptomyces sp.
Co2+ slight inhibition Streptomyces sp.
Fe2+ slight inhibition Streptomyces sp.
Fe3+ slight inhibition Streptomyces sp.
Hg+ complete inhibition Streptomyces sp.
Hg2+ complete inhibition Streptomyces sp.
Mn2+ slight inhibition Streptomyces sp.
p-chloromercuribenzoate complete inhibition Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
x * 20000, SDS-PAGE Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
M-20
-
Streptomyces sp. M-20
-
M-20
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces sp.

Renatured (Commentary)

Renatured (Comment) Organism
refolding after SDS-PAGE by the removal of SDS with purified Triton X-100 Streptomyces sp.

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
137
-
after Sephadex G-100 purification step Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O colloidal Streptomyces sp. ?
-
?
chitin + H2O colloidal Streptomyces sp. M-20 ?
-
?
N,N',N''-triacetylchitotriose + 2 H2O
-
Streptomyces sp. 3 N-acetyl-D-glucosamine
-
?
N,N',N''-triacetylchitotriose + 2 H2O
-
Streptomyces sp. M-20 3 N-acetyl-D-glucosamine
-
?

Subunits

Subunits Comment Organism
? x * 20000, SDS-PAGE Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
decrease in activity Streptomyces sp.
60
-
loss of activity Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Streptomyces sp.

pH Range

pH Minimum pH Maximum Comment Organism
4 8
-
Streptomyces sp.