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Literature summary for 3.2.1.14 extracted from

  • Genta, F.A.; Blanes, L.; Cristofoletti, P.T.; do Lago, C.L.; Terra, W.R.; Ferreira, C.
    Purification, characterization and molecular cloning of the major chitinase from Tenebrio molitor larval midgut (2006), Insect Biochem. Mol. Biol., 36, 789-800.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Tenebrio molitor

General Stability

General Stability Organism
unstable in the presence of midgut proteinases Tenebrio molitor

Inhibitors

Inhibitors Comment Organism Structure
chitobiose
-
Tenebrio molitor
chitotriose
-
Tenebrio molitor
methylumbelliferyl-beta-N',N',N'-triacetyl-chitotrioside
-
Tenebrio molitor
methylumbelliferyl-beta-N',N'-diacetyl-chitobioside
-
Tenebrio molitor
N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide
-
Tenebrio molitor
N-bromosuccinimide
-
Tenebrio molitor
p-hydroxymercuribenzoate
-
Tenebrio molitor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
methylumbelliferyl-beta-N',N'-diacetyl-chitobioside pH 5.0, 30°C Tenebrio molitor
0.023
-
N,N',N'',N''',N''''-pentaacetylchitopentaose pH 5.0, 30°C Tenebrio molitor
0.026
-
methylumbelliferyl-beta-N',N',N'-triacetyl-chitotrioside pH 5.0, 30°C Tenebrio molitor
0.05
-
methylumbelliferyl-beta-N'-acetylglucosamine pH 5.0, 30°C Tenebrio molitor
0.18
-
chitohexaose pH 5.0, 30°C Tenebrio molitor
0.98
-
chitotetraose pH 5.0, 30°C Tenebrio molitor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
gel filtration Tenebrio molitor
44000
-
1 * 44000, SDS-PAGE Tenebrio molitor

Organism

Organism UniProt Comment Textmining
Tenebrio molitor
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Tenebrio molitor

Source Tissue

Source Tissue Comment Organism Textmining
larva midgut Tenebrio molitor
-
midgut larva Tenebrio molitor
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.9
-
-
Tenebrio molitor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O low activity with colloidal chitin Tenebrio molitor ?
-
?
chitohexaose + H2O
-
Tenebrio molitor chitobiose + chitotriose
-
?
chitopentaose + H2O best substrate Tenebrio molitor chitobiose + chitotriose
-
?
chitotetraose + H2O
-
Tenebrio molitor 2 chitobiose
-
?
methylumbelliferyl-beta-N',N',N'-triacetyl-chitotrioside + H2O
-
Tenebrio molitor ?
-
?
methylumbelliferyl-beta-N',N'-diacetyl-chitobioside + H2O
-
Tenebrio molitor ?
-
?
methylumbelliferyl-beta-N'-acetylglucosamine + H2O
-
Tenebrio molitor ?
-
?
additional information TmChi does not hydrolyze chitobiose, and chitotriose Tenebrio molitor ?
-
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + 4 H2O
-
Tenebrio molitor 5 N-acetyl-D-glucosamine
-
?

Subunits

Subunits Comment Organism
monomer 1 * 44000, SDS-PAGE Tenebrio molitor

Synonyms

Synonyms Comment Organism
TmChi
-
Tenebrio molitor

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
300
-
methylumbelliferyl-beta-N'-acetylglucosamine pH 5.0, 30°C Tenebrio molitor
350
-
methylumbelliferyl-beta-N',N'-diacetyl-chitobioside pH 5.0, 30°C Tenebrio molitor
1500
-
methylumbelliferyl-beta-N',N',N'-triacetyl-chitotrioside pH 5.0, 30°C Tenebrio molitor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Tenebrio molitor

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.031
-
methylumbelliferyl-beta-N',N'-diacetyl-chitobioside pH 5.0, 30°C Tenebrio molitor
0.035
-
methylumbelliferyl-beta-N',N',N'-triacetyl-chitotrioside pH 5.0, 30°C Tenebrio molitor
0.04
-
chitobiose pH 5.0, 30° Tenebrio molitor
0.13
-
chitotriose pH 5.0, 30° Tenebrio molitor