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Literature summary for 3.2.1.14 extracted from
- Identification and characterization of a chitinase of Stenotrophomonas maltophilia, a bacterium that is antagonistic towards fungal phytopathogens (2011), J. Biosci. Bioeng., 113, 30-35.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Stenotrophomonas maltophilia |  |
| Cu2+ | - |
Stenotrophomonas maltophilia | |
| Hg2+ | - |
Stenotrophomonas maltophilia | |
| Zn2+ | - |
Stenotrophomonas maltophilia | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the enzyme is secreted | Stenotrophomonas maltophilia | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stabilizes the enzyme | Stenotrophomonas maltophilia | |
| Mg2+ | stabilizes the enzyme | Stenotrophomonas maltophilia | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
x * 52000, SDS-PAGE | Stenotrophomonas maltophilia |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Stenotrophomonas maltophilia | - |
isolated from the rhizosphere of winter wheat | - |
| Stenotrophomonas maltophilia MUJ | - |
isolated from the rhizosphere of winter wheat | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 5.8fold from culture supernatant by ammonium sulfate fractionation, ultrafiltration, and chitin affinity chromatography | Stenotrophomonas maltophilia |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 20.3 | - |
purified enzyme, pH 6.8, 45°C | Stenotrophomonas maltophilia |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 52000, SDS-PAGE | Stenotrophomonas maltophilia |
| More | enzyme peptide mapping, overview | Stenotrophomonas maltophilia |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| chitinolytic enzyme | - |
Stenotrophomonas maltophilia |
| More | the enzyme belongs to family 18 of glycosyl hydrolases | Stenotrophomonas maltophilia |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Stenotrophomonas maltophilia |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 55 | activity range, 80% of maximal activity at 40°C and 50°C, inactive at 60°C | Stenotrophomonas maltophilia |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
purified enzyme, 3 h, completely stable | Stenotrophomonas maltophilia |
| 45 | - |
purified enzyme, 3 h, loss of 15% activity | Stenotrophomonas maltophilia |
| 50 | - |
purified enzyme, 2 h, loss of 70% activity | Stenotrophomonas maltophilia |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Stenotrophomonas maltophilia |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8.5 | activity range, 10% of maximal activity at pH 5.5 and pH 8.5 | Stenotrophomonas maltophilia |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the purified enzyme inhibits the growth of fungal phytopathogens belonging to the genera Fusarium, Rhizoctonia and Alternaria | Stenotrophomonas maltophilia |
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