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Literature summary for 3.2.1.14 extracted from

  • Laribi-Habchi, H.; Dziril, M.; Badis, A.; Mouhoub, S.; Mameri, N.
    Purification and characterization of a highly thermostable chitinase from the stomach of the red scorpionfish Scorpaena scrofa with bioinsecticidal activity toward cowpea weevil Callosobruchus maculatus (Coleoptera: Bruchidae) (2012), Biosci. Biotechnol. Biochem., 76, 1733-1740.
    View publication on PubMed
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Application

Application Comment Organism
agriculture the enzyme might be used in biotechnological strategies of insect management for stored cowpeas Scorpaena scrofa

General Stability

General Stability Organism
Co2+ stabilizes the purified enzyme Scorpaena scrofa

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate complete inhibition Scorpaena scrofa
Hg+ complete inhibition Scorpaena scrofa
Hg2+ complete inhibition Scorpaena scrofa
N-ethylmaleimide complete inhibition Scorpaena scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Scorpaena scrofa
0.419
-
 colloidal chitin pH 5.0, 37°C Scorpaena scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Scorpaena scrofa
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ highl activating at 2 mM, Co2+ stabilizes the purified enzyme Scorpaena scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 gel filtration Scorpaena scrofa
50103
-
 1 * 50103, mass spectrometry, 1 * 50000, SDS-PAGE Scorpaena scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitin + H2O Scorpaena scrofa
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Scorpaena scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 162.75fold from stomach to homogeneity by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography Scorpaena scrofa

Source Tissue

Source Tissue Comment Organism Textmining
stomach
-
 Scorpaena scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.51
-
 purified enzyme, pH 5.0, 37°C, substrate colloidal chitin Scorpaena scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-N,N',N'',N'''-tetraacetylchitotetraoside + 3 H2O
-
 Scorpaena scrofa 4-nitrophenol + 2 N,N'-diacetylchitobiose
-
 ?
4-nitrophenyl beta-N,N',N''-triacetylchitotrioside + H2O
-
 Scorpaena scrofa ?
-
 ?
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
-
 Scorpaena scrofa 4-nitrophenol + N,N'-diacetylchitobiose
-
 ?
chitin + H2O
-
 Scorpaena scrofa ?
-
 ?
chitotriose + H2O
-
 Scorpaena scrofa ?
-
 ?
colloidal chitin + H2O
-
 Scorpaena scrofa ?
-
 ?
glycol chitin + H2O
-
 Scorpaena scrofa ?
-
 ?
glycol chitosan + H2O
-
 Scorpaena scrofa ?
-
 ?
additional information the enzyme hydrolyzes randomly beta-14,-N-acetylglucosamine linkages in the chitin polymer, and preferentially hydrolyzes the second glycosidic link from the nonreducing end of the N-acetylglucosamine oligomers Scorpaena scrofa ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 50103, mass spectrometry, 1 * 50000, SDS-PAGE Scorpaena scrofa

Synonyms

Synonyms Comment Organism
SsChi50
-
 Scorpaena scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Scorpaena scrofa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 100 over 50% activity in this range, profile overview Scorpaena scrofa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 purified enzyme, pH 5.0, with 2 mM Co2+: completely stable for 10 h, half-life is 18 h, about 10% activity remaining after 24 h, without Co2+: completely stable for 6 h, half-life is 14 h, inactivation after 20 h Scorpaena scrofa
80
-
 purified enzyme, pH 5.0, with 2 mM Co2+: completely stable for 4 h, half-life is 12 h, inactivation after 16 h, without Co2+: completely stable for 2 h, half-life is 10 h, inactivation after 18 h Scorpaena scrofa
90
-
 purified enzyme, pH 5.0, with 2 mM Co2+: completely stable for 2 h, half-life is 8 h, inactivation after 12 h, without Co2+: half-life is 6 h, inactivation after 14 h Scorpaena scrofa
100
-
 purified enzyme, pH 5.0, with 2 mM Co2+: half-life is 4 h, inactivation after 6 h, without Co2+: half-life is 2 h, inactivation after 8 h Scorpaena scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.33
-
 colloidal chitin pH 5.0, 37°C Scorpaena scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Scorpaena scrofa

pH Range

pH Minimum pH Maximum Comment Organism
2 11 activity range, profile overview Scorpaena scrofa

pH Stability

pH Stability pH Stability Maximum Comment Organism
2
-
 purified enzyme, 60°C, 48 h, loss of 25% activity Scorpaena scrofa
3 7 purified enzyme, 60°C, 48 h, completely stable at Scorpaena scrofa
8
-
 purified enzyme, 60°C, 48 h, loss of 20% activity Scorpaena scrofa
9
-
 purified enzyme, 60°C, 48 h, loss of 30% activity Scorpaena scrofa

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycosyl hydrolase family GH18 Scorpaena scrofa
physiological function the chitinase from the stomach of the red scorpionfish Scorpaena scrofa has bioinsecticidal activity toward cowpea weevil Callosobruchus maculatus Scorpaena scrofa