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Literature summary for 3.2.1.147 extracted from

  • Ohtsuru, M.; Hata, T.
    The interaction of L-ascorbic acid with the active center of myrosinase (1979), Biochim. Biophys. Acta, 567, 384-391.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ascorbic acid activates, enzyme is conformationally changed, no activation by analogs Brassica juncea

Inhibitors

Inhibitors Comment Organism Structure
2-methoxy-5-nitrotropone 1 mM, strong Brassica juncea
5,5'-dithiobis(2-nitrobenzoic acid)
-
Brassica juncea
p-mercuribenzoate 0.1 mM, strong inhibition Brassica juncea

Organism

Organism UniProt Comment Textmining
Brassica juncea
-
i.e. yellow mustard
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sinigrin + H2O
-
Brassica juncea D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
in presence of L-ascorbic acid Brassica juncea
55
-
in absence of L-ascorbic acid Brassica juncea

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50 about 45% of maximal activity at 25°C and at 50°C, in presence of 1 mM ascorbic acid Brassica juncea