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Literature summary for 3.2.1.15 extracted from

  • Ishii, S.; Yokotsuka, T.
    Purification and properties of endo-polygalacturonase from Aspergillus japonicus (1972), Agric. Biol. Chem., 36, 1885-1893.
No PubMed abstract available
Search for more literature for 3.2.1.15

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35500
-
 gel filtration Aspergillus japonicus

Organism

Organism UniProt Comment Textmining
Aspergillus japonicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus japonicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1362
-
-
 Aspergillus japonicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information maceration of plant tissues Aspergillus japonicus ?
-
 ?
pectic acid + H2O
-
 Aspergillus japonicus galacturonic acid oligomers
-
 ?
pectin + H2O
-
 Aspergillus japonicus ?
-
 ?
polygalacturonate + H2O
-
 Aspergillus japonicus oligogalacturonates higher galacturonides progressively disappear with reaction time, and monogalacturonic acid, digalacturonic acid and trigalacturonic acids remain as the end products of the hydrolysis ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 pH 4.5, stable below Aspergillus japonicus
65
-
 10 min, complete loss of activity Aspergillus japonicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 hydrolysis of pectin or pectic acid Aspergillus japonicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 5 4°C or 30°C, stable Aspergillus japonicus