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Literature summary for 3.2.1.15 extracted from
- Purification and characterization of polygalacturonase from banana fruit (2000), Phytochemistry, 54, 147-152.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | activates | Musca sp. |  |
| DTT | 40% activation by 1 mM, 180% activation by 5 mM | Musca sp. | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | 1 mM, 20% inhibition | Musca sp. | |
| PCMB | 45% inhibition by 0.1 mM, 65% inhibition by 0.5 mM. Inhibition can be reversed by DTT | Musca sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | KM-value for polygalacturonate is 0.15% | Musca sp. |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
x * 29000, SDS-PAGE | Musca sp. |
| 90000 | - |
gel filtration | Musca sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Musca sp. | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 8% carbohydrate | Musca sp. |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Musca sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | - |
Musca sp. | - |
| pulp | - |
Musca sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.75 | - |
- |
Musca sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polygalacturonate + H2O | - |
Musca sp. | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 29000, SDS-PAGE | Musca sp. |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PG-3 | - |
Musca sp. |
| polygalacturonase-3 | - |
Musca sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Musca sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.3 | - |
- |
Musca sp. |
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