Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain Origami (DE3) provides a soluble and active enzyme with proper disulfide bond formation, the enzyme expressed in BL21 is localized in inclusion bodies | Chondrostereum purpureum |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapor diffusion method at 20°C, pH 5.0 | Chondrostereum purpureum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | expression in Escherichia coli strain Origami (DE3) provides a soluble and active enzyme with proper disulfide bond formation, the enzyme expressed in BL21 is localized in inclusion bodies | Chondrostereum purpureum | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34632 | - |
x * 34632, ESI-MS | Chondrostereum purpureum |
34751 | - |
x * 34751, calculated from sequence | Chondrostereum purpureum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chondrostereum purpureum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Chondrostereum purpureum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
442 | - |
- |
Chondrostereum purpureum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
polygalacturonic acid + H2O | random hydrolysis of the alpha-1,4 glycosidic linkages | Chondrostereum purpureum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34632, ESI-MS | Chondrostereum purpureum |
? | x * 34751, calculated from sequence | Chondrostereum purpureum |
Synonyms | Comment | Organism |
---|---|---|
EndoPG I | - |
Chondrostereum purpureum |
endopolygalacturonase | - |
Chondrostereum purpureum |