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Literature summary for 3.2.1.15 extracted from
- Purification, characterization, kinetic properties, and thermal behavior of extracellular polygalacturonase produced by filamentous fungus Tetracoccosporium sp (2006), Appl. Biochem. Biotechnol., 135, 193-208.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Tetracoccosporium sp. |  |
| Triton X-100 | - |
Tetracoccosporium sp. | |
| Tween-20 | - |
Tetracoccosporium sp. | |
| Tween-80 | - |
Tetracoccosporium sp. | |
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | the polygalacturonase has a remarkable heat-tolerance, which makes it very attractive for industrial applications | Tetracoccosporium sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | - |
Tetracoccosporium sp. | |
| Ba2+ | - |
Tetracoccosporium sp. | |
| Ca2+ | - |
Tetracoccosporium sp. | |
| Fe2+ | - |
Tetracoccosporium sp. | |
| Fe3+ | - |
Tetracoccosporium sp. | |
| Mg2+ | - |
Tetracoccosporium sp. | |
| Mn2+ | - |
Tetracoccosporium sp. | |
| additional information | not inhibited by iodoacetamide and iodoacetic acid at a concentration of 1 mM | Tetracoccosporium sp. | |
| Ni2+ | - |
Tetracoccosporium sp. | |
| SDS | - |
Tetracoccosporium sp. | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Tetracoccosporium sp. | |
| Co2+ | - |
Tetracoccosporium sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
x * 36000, SDS-PAGE | Tetracoccosporium sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tetracoccosporium sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by ammonium sulfate precipitation and two-step anion-exchange chromatography | Tetracoccosporium sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.17 | - |
- |
Tetracoccosporium sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polygalacturonic acid + H2O | - |
Tetracoccosporium sp. | galacturonic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 36000, SDS-PAGE | Tetracoccosporium sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Tetracoccosporium sp. |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 70 | - |
Tetracoccosporium sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
halflife is 63.01 min | Tetracoccosporium sp. |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.894 | - |
polygalacturonic acid | - |
Tetracoccosporium sp. | |
| 2.94 | - |
polygalacturonic acid | - |
Tetracoccosporium sp. | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.3 | - |
- |
Tetracoccosporium sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | - |
Tetracoccosporium sp. |
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