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Literature summary for 3.2.1.15 extracted from

  • Saad, N.; Briand, M.; Gardarin, C.; Briand, Y.; Michaud, P.
    Production, purification and characterization of an endopolygalacturonase from Mucor rouxii NRRL 1894 (2007), Enzyme Microb. Technol., 41, 800-805.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 78% of activity reduction Amylomyces rouxii
Hg2+ 90.5% of activity reduction Amylomyces rouxii
Mg2+ reduces activity by 50% Amylomyces rouxii
additional information no considerable effect with Li+, Fe2+ and Rb2+ Amylomyces rouxii
Ni(2+) reduces activity by 50% Amylomyces rouxii

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ significantly increases activity Amylomyces rouxii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43100
-
gel filtration Amylomyces rouxii

Organism

Organism UniProt Comment Textmining
Amylomyces rouxii
-
-
-
Amylomyces rouxii NRRL 1894
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity by two chromatographic steps using cation exchange column and gel filtration, 85fold Amylomyces rouxii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.1
-
crude extract Amylomyces rouxii
1328
-
85fold purified enzyme Amylomyces rouxii

Storage Stability

Storage Stability Organism
-80°C Amylomyces rouxii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pectin + H2O citrus pectin, hydrolysis decreases with increasing of degrees of esterification Amylomyces rouxii ?
-
?
pectin + H2O citrus pectin, hydrolysis decreases with increasing of degrees of esterification Amylomyces rouxii NRRL 1894 ?
-
?
polygalacturonic acid + H2O
-
Amylomyces rouxii oligogalacturonates oligosaccharides with various polymerization degrees between 1 and 4 ?
polygalacturonic acid + H2O
-
Amylomyces rouxii NRRL 1894 oligogalacturonates oligosaccharides with various polymerization degrees between 1 and 4 ?

Subunits

Subunits Comment Organism
monomer 1 * 43100, SDS-PAGE Amylomyces rouxii

Synonyms

Synonyms Comment Organism
endopolygalacturonase
-
Amylomyces rouxii
PGase
-
Amylomyces rouxii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Amylomyces rouxii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
stable up to 30°C, exhibits 49% of its activity at 20°C, stability decreases rapidly above 60°C Amylomyces rouxii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Amylomyces rouxii

pI Value

Organism Comment pI Value Maximum pI Value
Amylomyces rouxii isoelectrofocusing
-
6