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Literature summary for 3.2.1.15 extracted from
- Biochemical characterization of an extracellular polygalacturonase from Trichoderma harzianum (2006), J. Biotechnol., 127, 54-64.
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | properties of the enzyme may be highly beneficial during fruit processing | Trichoderma harzianum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 56.6% residual activity with 1mM | Trichoderma harzianum |  |
| Ba2+ | 96.3% residual activity with 1mM | Trichoderma harzianum | |
| Ca2+ | 66.7% residual activity with 1mM | Trichoderma harzianum | |
| Citric acid | 64% inhibition at 5 mM | Trichoderma harzianum | |
| Co2+ | 1mM completely inhibits | Trichoderma harzianum | |
| Cu2+ | 83.6% residual activity with 1mM | Trichoderma harzianum | |
| Fe3+ | 93.2% residual activity with 1mM | Trichoderma harzianum | |
| Hg2+ | 46.3% residual activity with 1mM | Trichoderma harzianum | |
| iodoacetic acid | 74% inhibition at 5 mM | Trichoderma harzianum | |
| Li+ | 83.6% residual activity with 1mM | Trichoderma harzianum | |
| Mg2+ | 76.4% residual activity with 1mM | Trichoderma harzianum | |
| Mn2+ | 1mM completely inhibits | Trichoderma harzianum | |
| additional information | 1,10-phenanthroline, Tween 20, Tween 80, Triton X-100 and SDS have no effect on enzyme activity | Trichoderma harzianum | |
| Ni2+ | 89.9% residual activity with 1mM | Trichoderma harzianum | |
| PMSF | 78% inhibition at 5 mM | Trichoderma harzianum | |
| Zn2+ | 81.7% residual activity with 1mM | Trichoderma harzianum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
gel filtration | Trichoderma harzianum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichoderma harzianum | - |
- |
- |
| Trichoderma harzianum CECT 2413 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 13fold, to homogeneity by two chromatography steps | Trichoderma harzianum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 21 | - |
culture filtrate | Trichoderma harzianum |
| 276 | - |
13fold purified enzyme | Trichoderma harzianum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-4°C, stable for 3 months | Trichoderma harzianum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | very low activity toward non-pectic polysaccharides | Trichoderma harzianum | ? | - |
? | |
| additional information | very low activity toward non-pectic polysaccharides | Trichoderma harzianum CECT 2413 | ? | - |
? | |
| pectin + H2O | citrus pectin, apple pectin and amylopectin, hydrolysis of different pectins is decreased with increasing of degree of esterification | Trichoderma harzianum | ? | - |
? | |
| pectin + H2O | citrus pectin, apple pectin and amylopectin, hydrolysis of different pectins is decreased with increasing of degree of esterification | Trichoderma harzianum CECT 2413 | ? | - |
? | |
| polygalacturonic acid + H2O | - |
Trichoderma harzianum | ? | - |
? | |
| polygalacturonic acid + H2O | - |
Trichoderma harzianum CECT 2413 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 31000, SDS-PAGE | Trichoderma harzianum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endoPG | - |
Trichoderma harzianum |
| PGII | - |
Trichoderma harzianum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Trichoderma harzianum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
approximately stable up to 30°C for 60 min of incubation. Activity of enzyme is gradually decreased with increasing temperature and time | Trichoderma harzianum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 592 | - |
polygalacturonic acid | - |
Trichoderma harzianum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Trichoderma harzianum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 8 | - |
Trichoderma harzianum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
very stable at pH 5.0, retains 49% and 75% of its activity at pH 4.0 and 6.0, respectively. Half life time of enzymatic activity is 24 h at pH 4.0. The enzyme loses about 70-80% of its activity at pH 3.0 and pHs from 7.0 to 11 | Trichoderma harzianum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Trichoderma harzianum | isoelectric focusing | - |
4.5 |
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