Literature summary for 3.2.1.15 extracted from

Joubert, D.A.; Kars, I.; Wagemakers, L.; Bergmann, C.; Kemp, G.; Vivier, M.A.; van Kan, J.A.
A polygalacturonase-inhibiting protein from grapevine reduces the symptoms of the endopolygalacturonase BcPG2 from Botrytis cinerea in Nicotiana benthamiana leaves without any evidence for in vitro interaction (2007), Mol. Plant Microbe Interact., 20, 392-402.

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Application

Application	Comment	Organism
agriculture	PG1 causes chlorosis and the development of yellow or brown patches scattered over the infiltrated area of the leaf	Botrytis cinerea
agriculture	PG2 causes the most severe symptoms like necrosis and tissue collapse as compared to other PGs	Botrytis cinerea
agriculture	PG3 cause no symptoms	Botrytis cinerea
agriculture	PG4 causes chlorosis and the development of yellow or brown patches scattered over the infiltrated area of the leaf similar to PG1	Botrytis cinerea
agriculture	PG5 causes chlorosis and the development of yellow or brown patches scattered over the infiltrated area of the leaf similar to PG1	Botrytis cinerea
agriculture	PG6 cause no symptoms	Botrytis cinerea

Cloned(Commentary)

Cloned (Comment)	Organism
PG1 and mutated form of PG1 transiently expressed in leaves of Nicotiana benthamiana. Coexpression of pgg2 and the pgip1 gene from Vitis vinifera in Nicotiana benthamiana	Botrytis cinerea
PG2 and mutated form of PG2 transiently expressed in leaves of Nicotiana benthamiana. Coexpression of pgg2 and the pgip1 gene from Vitis vinifera in Nicotiana benthamiana. PG2-D192A mutant produced in Pichia pastoris	Botrytis cinerea
PG3 transiently expressed in leaves of Nicotiana benthamiana	Botrytis cinerea
PG4 transiently expressed in leaves of Nicotiana benthamiana	Botrytis cinerea
PG5 transiently expressed in leaves of Nicotiana benthamiana	Botrytis cinerea
PG6 transiently expressed in leaves of Nicotiana benthamiana	Botrytis cinerea

Protein Variants

Protein Variants	Comment	Organism
D192A	PG2 mutant causes no symptoms, lacks PG activity and is unable to cause symptoms in plant tissue upon infiltration	Botrytis cinerea
D203A	PG1 mutant, causes chlorotic symptoms with scattered yellow or brown patches to a similar extent as the wild-type PG1	Botrytis cinerea

Inhibitors

Inhibitors	Comment	Organism	Structure
polygalacturonase-inhibiting protein 1	coinfiltration of Vitis vinifera polygalacturonase-inhibiting protein 1 and PG2 results in a substantial reduction of the symptoms inflicted by the activity of PG2 in planta. In vitro, the inhibitor neither inhibits PG2 activity nor alters the degradation profile of polygalacturonic acid by PG2 and does not physically interact with PG2	Botrytis cinerea

Organism

Organism	UniProt	Comment	Textmining
Botrytis cinerea	Q4G495	-	-
Botrytis cinerea	Q4G496	-	-
Botrytis cinerea	Q9Y7V9	-	-
Botrytis cinerea	Q9Y7W0	-	-
Botrytis cinerea	Q9Y7W1	-	-
Botrytis cinerea	Q9Y7W2	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
polygalacturonic acid + H2O	-	Botrytis cinerea	?	-	?

Synonyms

Synonyms	Comment	Organism
endopolygalacturonase	-	Botrytis cinerea
PG1	-	Botrytis cinerea
PG2	-	Botrytis cinerea
PG3	-	Botrytis cinerea
PG4	-	Botrytis cinerea
PG5	-	Botrytis cinerea
PG6	-	Botrytis cinerea

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Release 2023.1 (January 2023)