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Literature summary for 3.2.1.15 extracted from
- Identification, characterization and regulation of two secreted polygalacturonases of the emerging rice pathogen Burkholderia glumae (2008), FEMS Microbiol. Ecol., 65, 251-262.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | pehB promoter is regulated by N-acyl homoserine lactone-dependent, cell-density-dependent quorum-sensing regulation system | Burkholderia glumae | |
| polygalacturonic acid | inducer | Burkholderia glumae |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into pBluescript KS | Burkholderia glumae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | when PehA is inactivated, Burkholderia glumae retains rice virulence comparable to that of the wild-type parent strain | Burkholderia glumae |
| additional information | when PehB is inactivated, Burkholderia glumae retains rice virulence comparable to that of the wild-type parent strain | Burkholderia glumae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
gel filtration | Burkholderia glumae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Burkholderia glumae | A8YPP2 | strain ATCC33617 and strain AU6208 | - |
| Burkholderia glumae | A8YPP3 | strain ATCC33617 and strain AU6208 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, by low-pressure liquid chromatography system, 54.4fold | Burkholderia glumae |
| to homogeneity, by low-pressure liquid chromatography system, 6.9fold | Burkholderia glumae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.9 | - |
supernatant | Burkholderia glumae |
| 3.5 | - |
supernatant | Burkholderia glumae |
| 24.2 | - |
6.9fold purified | Burkholderia glumae |
| 103.5 | - |
54.4fold purified | Burkholderia glumae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polygalacturonic acid + H2O | - |
Burkholderia glumae | D-galacturonic acid + di-galacturonic acid + tri-galacturonic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1* 47000, SDS-PAGE | Burkholderia glumae |
| monomer | 1* 50000, SDS-PAGE | Burkholderia glumae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endopolygalacturonase | - |
Burkholderia glumae |
| PehA | - |
Burkholderia glumae |
| PehB | - |
Burkholderia glumae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Burkholderia glumae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Burkholderia glumae |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Burkholderia glumae | - |
9.2 | 6.12 |
| Burkholderia glumae | - |
9.2 | 8.69 |
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Release 2023.1 (January 2023)
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