Literature summary for 3.2.1.15 extracted from

Do Rosario Freixo, M.; Karmali, A.; Arteiro, J.M.
Production of polygalacturonase from Coriolus versicolor grown on tomato pomace and its chromatographic behaviour on immobilized metal chelates (2008), J. Ind. Microbiol. Biotechnol., 35, 475-484.

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	amount of pectin in tomato pomace is high enough to obtain high levels of polygalacturonase. Production of polygalacturonase grown on pectin as a sole carbon source, increases with the time of cultivation	Trametes versicolor

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
30000	-	x * 63300, SDS-PAGE, x * 43000, SDS-PAGE, x * 30000, SDS-PAGE	Trametes versicolor
43000	-	x * 63300, SDS-PAGE, x * 43000, SDS-PAGE, x * 30000, SDS-PAGE	Trametes versicolor
63300	-	x * 63300, SDS-PAGE, x * 43000, SDS-PAGE, x * 30000, SDS-PAGE	Trametes versicolor

Organism

Organism	UniProt	Comment	Textmining
Trametes versicolor	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
one-step purification at pH 6.0 by immobilized metal affinity chromatography in the presence of 2 mM (4 mM) imidazole in the equilibration buffer, final recovery of enzyme activity of 100% (50%), about 10fold (12fold) purified. 5.3 and 3.4fold purified at pH 6.0 and 8.0 on affinity column. Purification factors of 7.2 and 5.3fold by using immobilized metal chelates containing short and long spacer arms, respectively	Trametes versicolor

Purification (Comment)

Organism

one-step purification at pH 6.0 by immobilized metal affinity chromatography in the presence of 2 mM (4 mM) imidazole in the equilibration buffer, final recovery of enzyme activity of 100% (50%), about 10fold (12fold) purified. 5.3 and 3.4fold purified at pH 6.0 and 8.0 on affinity column. Purification factors of 7.2 and 5.3fold by using immobilized metal chelates containing short and long spacer arms, respectively

Trametes versicolor

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
14.5	-	grown on tomato pomace, third day of culture	Trametes versicolor
147	-	enzyme 9.97fold purified, in the presence of 2 mM imidazole	Trametes versicolor
178.2	-	enzyme 12.34fold purified, in the presence of 4 mM imidazole	Trametes versicolor
248	-	grown on pectin as a sole carbon source	Trametes versicolor

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
pectin + H2O	-	Trametes versicolor	oligogalacturonides	-	?
polygalacturonic acid + H2O	-	Trametes versicolor	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 63300, SDS-PAGE, x * 43000, SDS-PAGE, x * 30000, SDS-PAGE	Trametes versicolor

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	8	-	Trametes versicolor