Literature summary for 3.2.1.15 extracted from

Ogawa, S.; Shimizu, T.; Kimura, T.; Utoh, K.; Okuno, T.; Miyairi, K.
The pro-form of Stereum purpureum endopolygalacturonase I is inactivated by a pro-sequence in the C-terminal region (2010), Biosci. Biotechnol. Biochem., 74, 558-562.

Search for more literature for 3.2.1.15

Cloned(Commentary)

Cloned (Comment)	Organism
the pro-form of polygalacturonan I is expressed in Escherichia coli Origami (DE3) cells	Chondrostereum purpureum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
polygalacturonate + H2O	Chondrostereum purpureum	-	oligogalacturonates	-	?

Organism

Organism	UniProt	Comment	Textmining
Chondrostereum purpureum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography, gel filtration	Chondrostereum purpureum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + H2O	-	Chondrostereum purpureum	alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-D-galacturonate + D-galacturonate	-	?
polygalacturonate + H2O	-	Chondrostereum purpureum	oligogalacturonates	-	?

Synonyms

Synonyms	Comment	Organism
EndoPG I	-	Chondrostereum purpureum
endopolygalacturonase I	-	Chondrostereum purpureum

Expression

Organism	Comment	Expression
Chondrostereum purpureum	the C-terminal region (pro-sequence) of the pro-form of EndoPG I is responsible for the inactivation of polygalacturonan activity	down

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Release 2023.1 (January 2023)