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Literature summary for 3.2.1.15 extracted from

  • Martos, M.; Butiuk, A.; Rojas, N.; Hours, R.
    Purification and characterization of a polygalacturonase produced by Wickerhamomyces anomalus (2014), Braz. Arch. Biol. Technol., 57, 587-594.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
K+
-
Wickerhamomyces anomalus
Mg2+
-
Wickerhamomyces anomalus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Wickerhamomyces anomalus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Cu2+, Fe3+, Mg2+, Mn2+, and Zn2+ do not show any significant effect on the enzyme activity Wickerhamomyces anomalus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
x * 43000, SDS-PAGE Wickerhamomyces anomalus

Organism

Organism UniProt Comment Textmining
Wickerhamomyces anomalus
-
isolated from the citrus fruit peels in the province of Misiones, Argentina
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from culture supernatant to homogeneity by anion exchange chromatography, gel filtration, and dialysis, and cation exchange chromatography Wickerhamomyces anomalus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
258
-
partially purified enzyme, pH 5.0, 30°C Wickerhamomyces anomalus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the purified enzyme is able to macerate cassava tissues Wickerhamomyces anomalus ?
-
?
polygalacturonic acid + H2O
-
Wickerhamomyces anomalus ?
-
?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Wickerhamomyces anomalus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Wickerhamomyces anomalus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37 49 purified enzyme, pH 3.0-6.5, 10 h, stable at Wickerhamomyces anomalus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2
-
-
Wickerhamomyces anomalus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 6.5 purified enzyme, 49°C, 10 h, stable at Wickerhamomyces anomalus