Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Rhizomucor pusillus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
1 * 32000, SDS-PAGE | Rhizomucor pusillus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
polygalacturonic acid + H2O | Rhizomucor pusillus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizomucor pusillus | - |
isolated from decomposting orange peels | - |
Purification (Comment) | Organism |
---|---|
native enzyme 12.34fold by two different steps of gel filtration, to homogeneity | Rhizomucor pusillus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
32.57 U/ml for crude enzyme and 61.35 U/ml for purified enzyme, pH 5.0, 55°C | Rhizomucor pusillus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellulose + H2O | 37.8% activity compared to polygalacturonic acid | Rhizomucor pusillus | ? | - |
? | |
pectin + H2O | 57% activity compared to polygalacturonic acid | Rhizomucor pusillus | ? | - |
? | |
polygalacturonic acid + H2O | - |
Rhizomucor pusillus | ? | - |
? | |
polygalacturonic acid + H2O | best substrate | Rhizomucor pusillus | ? | - |
? | |
xylan + H2O | 11% activity compared to polygalacturonic acid | Rhizomucor pusillus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 32000, SDS-PAGE | Rhizomucor pusillus |
Synonyms | Comment | Organism |
---|---|---|
PGase | - |
Rhizomucor pusillus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Rhizomucor pusillus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | 27% and 13% of maximal activity at 30°C and 70°C, resepctively | Rhizomucor pusillus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified enzyme, pH 4.0-5.0, 120 min, stable, rapid inactivation above | Rhizomucor pusillus |
60 | - |
above, purified enzyme, pH 5.0, 48.2% activity remaining after 10 min | Rhizomucor pusillus |
70 | - |
above, purified enzyme, pH 5.0, 9.3% activity remaining after 10 min | Rhizomucor pusillus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Rhizomucor pusillus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
above, purified enzyme, 60°C, rapid inactivation | Rhizomucor pusillus |